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Preparation method of polyhedral composite nano-micelle

A technology of nano-micelle and nano-composite glue, which is applied in the field of preparation of polyhedral composite micelles, and can solve the problems of limited assembly control, limited development and application, etc.

Active Publication Date: 2018-08-17
ZHONGYUAN ENGINEERING COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on the assembly control of this protein is very limited, which also limits its development and application in related fields to a certain extent.

Method used

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  • Preparation method of polyhedral composite nano-micelle

Examples

Experimental program
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Effect test

Embodiment 1

[0019] A preparation method of polyhedral composite nano micelles, comprising the following steps:

[0020] ① The method of extracting silk fibroin with a certain molecular weight is to use degummed silk to prepare it through a 9M LiBr·H2O dissolution system at a temperature of 80°C, and use a Sartocon Slice 200 tangential flow ultrafiltration system for ultrafiltration. The molecular weight cut-off of the membrane capsule is 10KDa. After ultrafiltration, use a dialysis bag with a molecular weight cut-off of 1KD to carry out dialysis in deionized water, and perform high-speed centrifugation of the obtained four-degree protein solution in a high-speed centrifuge at 12,000 rpm / min, and pass the obtained silk fibroin through The low-temperature freeze-drying mechanism is ready for use. Some samples were detected by polypropylene gel electrophoresis and high performance liquid chromatography. Its molecular weight was determined to be 56435Da, and its isoelectric point was 4.52. ...

Embodiment 2

[0026] ① The method of extracting silk fibroin with a certain molecular weight is to use degummed mulberry silk to prepare it through a 9M LiBr·H2O dissolution system at a temperature of 70°C and use a Sartocon Slice 200 tangential flow ultrafiltration system for ultrafiltration. The molecular weight cut-off of the membrane capsule is 10KDa. After the ultrafiltration is completed, use a dialysis bag with a molecular weight cut-off of 1KD to perform dialysis in deionized water. The obtained silk fibroin solution in this range is subjected to high-speed centrifugation at 25,000 rpm / min in a high-speed centrifuge, and the obtained silk fibroin is passed through The low-temperature freeze-drying mechanism is ready for use. Some samples were detected by polypropylene gel electrophoresis and high performance liquid chromatography. Its molecular weight was determined to be 62884Da, and its isoelectric point was 4.71.

[0027] ② The molecular weight of the α-casein used is 23600Da, ...

Embodiment 3

[0032]① The method of extracting silk fibroin with a certain molecular weight is to use degummed mulberry silk to prepare it through a 9M LiBr·H2O dissolution system at a temperature of 65°C and use a Sartocon Slice 200 tangential flow ultrafiltration system for ultrafiltration. The molecular weight cut-off of the membrane capsule is 10KDa. After ultrafiltration, use a dialysis bag with a molecular weight cut-off of 1KD to perform dialysis in deionized water, and perform high-speed centrifugation of the obtained silk fibroin solution in a high-speed centrifuge at 20,000rpm / min), and the obtained silk fibroin Use a low-temperature freeze-drying mechanism for later use. Some samples were detected by polypropylene gel electrophoresis and high performance liquid chromatography. Its molecular weight was determined to be 68552Da, and its isoelectric point was 4.56.

[0033] ② The molecular weight of the α-casein used is 23600Da, which comes from milk, and its isoelectric point is ...

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Abstract

The invention discloses a preparation method of polyhedral composite nano-micelle. The technical problem to be solved is that assembly control study on protein of biparental block copolymer is very limited. The preparation method comprises the following steps: (1) preparing silk fibroin with a molecular weight range of 56435-68552 Da and alpha-casein with the molecular weight of 23600 Da; (2) respectively preparing the silk fibroin and the alpha-casein into solutions with mass concentrations of 0.25-0.35 percent; (3) mixing the silk fibroin solution and the alpha-casein solution prepared in step (2) according to a mass ratio between 8 to 1 and 5 to 1, performing ultrasound treatment for 30-45min, injecting the mixture into a reactor of a synthesis device, and continuously assembling for 6-18 hours; (4) adding a fixing cross-linking agent, and continuously fixing to obtain the polyhedral composite nano-micelle. By adopting the method, the silk fibroin and the alpha-casein with certain molecular weights are taken as raw materials to prepare the polyhedral composite micelle for the first time.

Description

technical field [0001] The invention relates to the field of preparation of polymer nanomaterials, and its direction is to prepare nanomaterials with polyhedral appearance and performance characteristics, which can be used for specific optical nanomaterials, nanomolecular switches or drug slow-release materials administered at specific functional parts, specifically related to a A preparation method of polyhedral composite micelles. Background technique [0002] In recent years, polymer self-assembly has received widespread attention, and the research on the assembly mechanism and related applications of block copolymers has been in-depth, such as PLA-b-PEO, PS-b-PAA, PS-b-PEO , PS-b-PVP, PCEMA-b-PAA, PB-b-PEO, PCL-b-PEO, PBO-b-PEO system. By controlling the assembly conditions, they can interact and assemble to form self-assembled bodies with shapes such as spheres, cylinders, sheets, vesicles, and helices. Its research results can be applied to many fields, such as biome...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/107A61K47/42
CPCA61K9/1075A61K47/42
Inventor 苏小舟栗蕾陈劲松
Owner ZHONGYUAN ENGINEERING COLLEGE
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