Horse source tetanus antibody

Abstract

The invention relates to a horse source tetanus antibody. Horses which are free of natural antibodies and at appropriate ages, weights and good health states are selected, the horses are immunized with different dosages of tetanus sub-toxin fragments or mixed antigen of tetanus toxins, and then the antibody can be generated. The antibody has the beneficial effects that due to high immunogenicity of tetanus sub-toxins, the horse source tetanus antibody can be generated, can be massively produced without limits, is easy in quality control and is completely free of toxicity; and the antibody provided by the invention has an effect equivalent to that of a horse source tetanus antibody generated from tetanus toxin immunization.

Description

technical field [0001] The invention belongs to the technical field of immunology, and in particular relates to the use of tetanus subtoxin to immunize horses to produce equine tetanus antibodies. Background technique [0002] Tetanus is a serious infectious disease with a mortality rate as high as 20%-40%. Any trauma has the potential to develop tetanus. Tetanus antibody is the rapid and effective medicine of short-term prevention and treatment tetanus, and the tetanus treatment drug on the market at present has tetanus equine antitoxin (TAT) and tetanus human immunoglobulin (TIG). TIG is a specific immunoglobulin preparation extracted from plasma with high titer of tetanus antibody collected from blood donors immunized with tetanus toxoid after immunization with hepatitis B vaccine. TIG does not require a skin test and can be injected directly. However, because it is a blood product, there is a potential risk of infection with infectious diseases such as hepatitis C and ...

AI Technical Summary

Problems solved by technology

At present, there are still some problems in the preparation of TAT: (1) During tetanus booster immunization in horses, it is difficult to produce highly specific antibodies due to the complex composition of tetanus toxoid; (2) Toxoid still has certain toxicity, often high After a dose of tetanus toxoid antigen injection, the blood collection will gradually cause degeneration and necrosis of the liver cells of the horse. Generally, the immune blood collection begins after the tenth stage of the horse, and the liver enlarges and degenerates, turning into a "tofu dregs" shape, and finally the edge of the liver ruptures. , resulting in the death of the horse

Although its immune effect is better, there are still some problems: (1) Clostridium tetani can form spores, which is highly toxic and has certain dangers during production; It is easy to cause side effects; (3) Toxoids after chemical treatment may also undergo toxicity reversal, causing toxicity to horses

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