Extraction method of active components in isodon amethystoides(Benth)Cy Wu et Hsuan as well as application of active components in agriculture

An extraction method and technology of active ingredients are applied in the field of botanical pesticides to achieve the effect of inhibiting proliferation

Active Publication Date: 2018-08-24
安徽隆跃农业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In view of this, the purpose of the present invention is to provide a method for extracting the active components of Jujube japonica, the extracted active components have a good control effect on fungal diseases in agricultural production, and solve the health, environment and environmental problems caused by the current use of chemical pesticides. ecological issues

Method used

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  • Extraction method of active components in isodon amethystoides(Benth)Cy Wu et Hsuan as well as application of active components in agriculture
  • Extraction method of active components in isodon amethystoides(Benth)Cy Wu et Hsuan as well as application of active components in agriculture
  • Extraction method of active components in isodon amethystoides(Benth)Cy Wu et Hsuan as well as application of active components in agriculture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] After the dried king jujube cotyledons were crushed into 200 mesh particles on an ultrafine pulverizer, they were mixed with ethanol at a material-to-liquid ratio of 1kg:3L (m / V), and subjected to four-stage microwave extraction to obtain the active ingredients of the king jujube. The specific process As shown in Table 1:

[0038] Table 1. The extraction process of the ethanol extract of Wang Zaozi

[0039]

[0040] The active ingredients of Wangzaozi obtained in the examples were determined by HPLC, and the active ingredient content is shown in Table 2:

[0041] Table 2. Effective ingredients and content of ethanol extract of Wangzaozi

[0042]

[0043]

[0044] Inoculate Pyricularia grisea and Pyricularia oryzae on PDA solid medium, culture for 5 days in the dark at a constant temperature of 25℃, after the hyphae grow evenly, punch the PDA medium filled with hypha by a 0.5cm puncher, and inoculate them respectively On the PDA solid medium containing 13.3, 10, 5, 2.5, 1, 0.25...

Embodiment 2

[0049] After the dried king jujube cotyledons were crushed into 200 mesh particles on an ultrafine pulverizer, they were mixed with acetone at a material-to-liquid ratio of 1kg:3L (m / V), and subjected to four-stage microwave extraction. The specific process is shown in Table 4:

[0050] Table 4. The extraction process of acetone extract of Wangzaozi

[0051]

[0052] Pear anthracnose, penicillium, black spot pathogen and rice blast fungus were inoculated on PDA solid medium and cultured in the dark at 25℃ for 5 days. After the hyphae grew evenly, the 0.5cm punch was punched to cover the hyphae The PDA medium was inoculated on the PDA solid medium containing 13.3, 10, 5, 2.5, 1, 0.25, 0.1, 0.05 mg / mL of the extract of Example 2 respectively. Incubate at 25°C for 7 days and record the inhibition zone. The inhibition rate is shown in Table 5:

[0053] Table 5. Antibacterial rate of acetone extract of Wangzaozi

[0054]

[0055] It can be seen that the acetone extract of Wangzaozi has...

Embodiment 3

[0057] After the dried king jujube cotyledons were crushed into 200 mesh particles on an ultrafine pulverizer, they were mixed with chloroform at a material-to-liquid ratio of 1kg:3L (m / V), and then subjected to four-stage microwave extraction. The specific process is shown in Table 6:

[0058] Table 6. Extraction process of chloroform extract of Wang Zaozi

[0059]

[0060] Inoculate Aspergillus niger, Pear rotifer, Pear anthracnose, Penicillium, Pear black spot and Pyricularia oryzae on PDA solid medium, culture for 5 days in the dark at a constant temperature of 25°C, and 0.5 cm after the hyphae grows evenly. The PDA medium filled with hyphae was perforated by the hole tool and inoculated on the PDA solid medium containing 13.3, 10, 5, 2.5, 1, 0.25, 0.1, 0.05 mg / mL of the extract of Example 3, respectively. Incubate at 25°C for 7 days and record the inhibition zone. The inhibition rate is shown in Table 7:

[0061] Table 7. Antibacterial rate of chloroform extract of Wang Zaozi

...

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Abstract

The invention provides an extraction method of active components in isodon amethystoides(Benth)Cy Wu et Hsuan as well as application of extracts, obtained by the extraction method, in preparation of medicines for inhibiting the activity of agricultural pathogenic fungi, belonging to the technical field of botanical pesticide. The extraction method provided by the invention comprises the steps of crushing the isodon amethystoides(Benth)Cy Wu et Hsuan, mixing the crushed product with an organic solvent, and carrying out microwave treatment in four stages to obtain the extracts; the obtained extracts have certain antibacterial and bactericidal abilities against the agricultural pathogenic fungi such as alternaria kikuchiana, pear ring rot, pear anthracis, magnaporthe oryzae, penicillium and aspergillus niger, and can be applied to the preparation of medicines for preventing and controlling the corresponding pathogenic bacteria. The extraction method is simple and rapid, wide in raw material source, free from special requirements for instruments, and suitable for large-scale production.

Description

Technical field [0001] The invention belongs to the field of plant-derived pesticides, and particularly relates to an extraction method of a king jujube extract and the application of the extract in agriculture. Background technique [0002] Diseases are one of the main factors affecting agricultural production, and the annual loss of production is reduced by 10% to 15% of the total output, of which more than 70% are caused by pathogenic fungi. For a long time, chemical pesticides have been the main or even the only way to control fungal diseases. The long-term application of chemical pesticides poses serious safety risks to human health and the ecological environment. Finding safe and efficient fungal disease prevention methods is an urgent problem in current agricultural production. Botanical pesticides use active substances produced by plant cells or plant tissues as pesticides. They have the advantages of wide sources of raw materials, low toxic and side effects, safety to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/22A01N45/00A01P3/00
CPCA01N45/00A01N65/00A01N65/22
Inventor 段永波赵丰兰薛涛滕井通张婉君朱艳芳盛玮张爱民薛建平
Owner 安徽隆跃农业发展有限公司
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