Film clipping device with compression positioning and guide clipping functions and application method of film clipping device

Abstract

The invention provides a film clipping device with compression positioning and guide clipping functions and an application method of the film clipping device. The film clipping device comprises an upper plate, a lower plate and a clipper, axle wires of the upper plate and the lower plate are coincident, a hinge shaft is arranged at the joint of the upper plate and the lower plate, the upper plateand the lower plate are rotationally connected through the hinge shaft, a strip-shaped guide rail groove is formed in the plate face of the upper plate in the film clipping direction, the clipper is arranged in the guide rail groove and slide along the guide rail groove, a strip-shaped cutting groove is formed in the plate face of the lower plate in the film clipping direction, and the guide groove is matched with the cutting groove. By means of the film clipping device, accurate film clipping can be achieved, the accuracy and the regularity are improved, operation difficulty is reduced, operation of the clipping film becomes easy to implement for a green hand, interference of human factors in clipping accuracy is reduced, influences of inaccurate operation caused by insufficient shear clipping experience traditionally are avoided, and therefore the equipment is worthy of wide application and popularization, and the working efficiency is improved.

Description

technical field [0001] The invention relates to the field of film applications, in particular to a film cutting device with the functions of pressing, positioning and guiding cutting and an application method thereof. Background technique [0002] In the Western blot experiment, nitrocellulose membrane (nitrocellulose, NC) and polyvinylidene fluoride membrane (Polyvinylidene fluoride, PVDF) are generally selected. Nitrocellulose membrane (NC membrane) is the standard solid support for Western blotting experiments. In the environment of low ion transfer buffer, most negatively charged proteins will combine with nitrocellulose membrane with high affinity through hydrophobic interaction, and it is easy to block non-specific binding, so it has been widely used. Bound proteins can also be eluted with non-ionic detergents. According to the molecular weight of the protein to be transferred, nitrocellulose membranes (NC membranes) with different pore sizes should be selected. Bec...

AI Technical Summary

Problems solved by technology

However, cutting with scissors does not guarantee the regularity of PVDF / NC. When it is necessary to cut and separate two protein bands with similar molecular weights (the distance between the bands on the PVDF / NC membrane is relatively close), it is easy to cut to the required Protein bands will cause the experiment to fail, especially for novices, the operation will be more difficult, and it will also affect the accuracy and standardization of the experiment, which will affect the normal and orderly progress of the experiment

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