An anti-ricin immunoglobulin f(ab') 2 and its preparation method

A technology of immunoglobulin and ricin, applied in the direction of anti-plant immunoglobulin, immunoglobulin, immunoglobulin from serum, etc., can solve problems that are easy to cause social harm and difficult to supervise
CN108484760BActive Publication Date: 2021-08-31THE FIFTH MEDICAL CENT OF CHINESE PLA GENERAL HOSPITAL +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
THE FIFTH MEDICAL CENT OF CHINESE PLA GENERAL HOSPITAL
Publication Date
2021-08-31

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Abstract

The invention discloses an anti-ricin immunoglobulin F(ab')2 and a preparation method thereof. The invention provides a method for preparing anti-ricin immunoglobulin F(ab')2, comprising the steps of: immunizing animals with an immunogen, preparing ricin IgG antibodies, and then removing the ricin IgG antibodies The Fc segment obtains the anti-ricin immunoglobulin F (ab') 2; the antigen of the immunogen is as follows: 1), low toxicity or attenuated ricin mutant or its recombinant protein; 2) encoding the A DNA molecule of a low-toxic or attenuated ricin mutant or its recombinant protein, or a vector, an expression cassette, a recombinant bacterium or a recombinant virus expressing the DNA molecule; 3) detoxified natural ricin. The invention utilizes the non-toxic or low-toxic ricin antigen to immunize healthy animals to produce high titer neutralizing ricin antiserum, which has a specific protective effect on the treatment and prevention of the ricin.
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Description

technical field

[0001] The invention belongs to the field of biopharmaceuticals and relates to an anti-ricin immunoglobulin F (ab') 2 and its preparation method. Background technique

[0002] Ricin (ricin) is a highly toxic glycoprotein derived from plant castor seeds. The intraperitoneal median lethal dose (LD50) of ricin to mice is about 3-8 μg / kg, and the lethal dose to adults is about 150μg, its toxicity is equivalent to about 6000 times that of cyanide. Ricin is a highly toxic plant protein with two peptide chains, which can easily damage liver, kidney and other solid organs, causing bleeding, degeneration and necrosis. It can agglutinate and dissolve red blood cells, inhibit and paralyze the cardiovascular and respiratory centers, and is one of the main causes of death. The source of ricin is easy, easy to extract, easy to obtain, difficult to supervise, and easy to cause social harm. There have been many terrorist incidents using ricin in history. The 1993 Chemica...

Claims

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