High-flux continuous flow analysis method for water-soluble sugar in tobacco gene editing material

A gene editing, water-soluble sugar technology, applied in the field of tobacco chemistry research, can solve problems such as inconvenience, affect the efficiency of detection work, affect the reliability of detection results, etc., to speed up the process, improve the efficiency of sample analysis, and simplify the sample pretreatment process. Effect

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A gene editing, water-soluble sugar technology, applied in the field of tobacco chemistry research, can solve problems such as inconvenience, affect the efficiency of detection work, affect the reliability of detection results, etc., to speed up the process, improve the efficiency of sample analysis, and simplify the sample pretreatment process. Effect

CN108519338AInactive Publication Date: 2018-09-11CHINA TOBACCO YUNNAN IND

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Examples

Experimental program

Comparison scheme

Effect test

Embodiment 1

[0031] Embodiment 1: Determination of total sugar in flue-cured tobacco sample

[0032] 1) Sample pretreatment: firstly dry the tobacco leaves (or shredded tobacco), use a high-speed cyclone mill to break the cell walls of tobacco and tobacco products, then pass through a 60-mesh sieve, seal and store as a sample preparation. Test the water content of the sample according to the YC / T31-1996 standard, and then handle it as follows:

[0033] A. Accurately weigh 0.25g of sample into a 25mL improved sample tube, remove the sample tray from the automatic sampler of the continuous flow analyzer, and fill the sample tube with the weighed sample in order to inject into the continuous flow analyzer 100 sampling tube positions on the tray;

[0034] B. Use a 25mL pipette gun to add 25mL of 5% acetic acid solution to each sample tube, and then insert the inner casing of the filter sieve plate with a pore size of 0.45 μm at the bottom to prevent the solution from splashing during the vibr...

Embodiment 2

[0039] Embodiment 2: Determination of reducing sugar in flue-cured tobacco sample

[0040] Repeat the process in Example 1 to measure the reducing sugar in the flue-cured tobacco sample, and the measured reducing sugar content is 20.52%.

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Abstract

The invention discloses a high-flux continuous flow analysis method for water-soluble sugar in a tobacco gene editing material. The high-flux continuous flow analysis method comprises the following steps: A, accurately weighing 0.25g of a sample containing the water-soluble sugar and putting the sample into a 25mL sample tube; putting sample feeding tubes filled with the weighed sample on a samplefeeding disc of an automatic sample feeding device of a continuous flow analyzer in sequence until the sample feeding disc is fully filled with the sample feeding tubes; B, adding 25mL of a 5 percentacetic acid solution into each sample tube by utilizing a pipette; then inserting inner sleeves with filtering sieve plates with the pore diameter of 0.45mu m at the bottom in sequence, so as to prevent the solution from being splashed in an oscillation and extraction process; after inserting the inner sleeves, putting the sample disc with the inserted inner sleeves onto an oscillator and carrying out oscillation and extraction; C, after finishing the extraction, pressing the inner sleeves and introducing an extracted solution into the inner sleeves through the filtering sieve plates; then putting the sample disc on the automatic sample feeding device of the continuous flow analyzer and only inserting a sample feeding needle of the automatic sample feeding device into supernatant in the inner sleeve each time to suck a filtered sample; then transferring the filtered sample into the continuous flow analyzer and analyzing.

Description

technical field [0001] The invention belongs to the field of tobacco chemistry research, and specifically relates to a high-throughput analysis method suitable for detecting the content of water-soluble sugar in tobacco gene editing materials. Background technique [0002] With the advancement of the tobacco industry's "Tobacco Genome Project Major Project-Based Genome Editing Technology Factory Breeding Program", all gene-edited materials will be screened through chemical composition analysis, and the compatibility test of tobacco leaves and cigarette formula will be passed. Verification, to determine the varieties with strong industrial usability, has become a key technical content urgently needed by the tobacco industry. [0003] Water-soluble sugar is an important conventional chemical component in tobacco and an important indicator for tobacco quality evaluation. Currently, continuous flow analysis is commonly used in the detection of water-soluble sugars in tobacco. ...

Claims

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Application Information

Patent Timeline

11 Sep 2018

Publication

CN108519338A

IPC: G01N21/31; G01N21/78

CPC: G01N21/31; G01N21/78

Inventors: 李雪梅; 黄海涛