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A marker for breast cancer diagnosis and its screening method

A marker, breast cancer technology, applied in the direction of biochemical equipment and methods, microbial determination/examination, DNA/RNA fragments, etc., can solve the problem of low sensitivity of early breast cancer detection, and unsatisfactory sensitivity and specificity of early diagnosis And other issues

Active Publication Date: 2022-08-02
JIANGSU CANCER HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current methods of clinical diagnosis of breast cancer mainly rely on physical examination and imaging examination, but these methods have low sensitivity for early breast cancer detection, and the sensitivity and specificity of serum markers commonly used to diagnose breast cancer are low for early diagnosis. not ideal

Method used

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  • A marker for breast cancer diagnosis and its screening method
  • A marker for breast cancer diagnosis and its screening method
  • A marker for breast cancer diagnosis and its screening method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. Target tRF&tiRNA screening

[0032] 1) Tissue specimens (cancer and paracancerous) of 6 breast cancer patients were obtained from the biobank, and the Agilent BioAnalyzer 2100 (Agilent BioAnalyzer 2100) library preparation, Illumina NextSeq 500 high-throughput desktop next-generation sequencing system and high-throughput sequencing were used. Gene Expression Omnibus big data analysis screened tRFs & tiRNAs, the results showed that there were 1135 tRFs & tiRNAs differentially expressed by more than 2 times in tissues (496 up-regulated and 639 down-regulated, such as figure 2 shown);

[0033] 2) According to the difference fold FC (Fold change)>2, P image 3 shown);

[0034] 3) With FC>2.5, P<0.05, combined with Gene Ontology functional enrichment analysis (Gene Ontology functional enrichment analysis) and Kyoto Encyclopedia of Genes and Genomes pathway analysis (Kyoto Encyclopedia of Genes and Genomes pathway analysis) and breast cancer correlation Literature search...

Embodiment 2

[0046] 1. Target tRF&tiRNA screening

[0047] 1) Tissue specimens (cancer and adjacent cancer) of 6 breast cancer patients were obtained from the biobank, and the Agilent BioAnalyzer 2100 (Agilent BioAnalyzer 2100) library preparation, Illumina NextSeq 500 high-throughput desktop next-generation sequencing system and high-throughput sequencing were used. Gene Expression Omnibus big data analysis screened tRFs&tiRNAs, the results showed that there were 1135 tRFs&tiRNAs differentially expressed by more than 2 times in tissues (496 up-regulated and 639 down-regulated, such as figure 2 shown);

[0048] 2) According to the difference fold FC (Fold change)>2, P image 3 shown);

[0049] 3) With FC>2.5, P<0.05, combined with Gene Ontology functional enrichment analysis (Gene Ontology functional enrichment analysis) and Kyoto Encyclopedia of Genes and Genomes pathway analysis (Kyoto Encyclopedia of Genes and Genomes pathway analysis) and breast cancer correlation Literature search, ...

Embodiment 3

[0062] 1. Target tRF&tiRNA screening

[0063] 1) Tissue specimens (cancer and adjacent cancer) of 6 breast cancer patients were obtained from the biobank, and the Agilent BioAnalyzer 2100 (Agilent BioAnalyzer 2100) library preparation, Illumina NextSeq 500 high-throughput desktop next-generation sequencing system and high-throughput sequencing were used. Gene Expression Omnibus big data analysis screened tRFs&tiRNAs, the results showed that there were 1135 tRFs&tiRNAs differentially expressed by more than 2 times in tissues (496 up-regulated and 639 down-regulated, such as figure 2 shown);

[0064] 2) According to the difference fold FC (Fold change)>2, P image 3 shown);

[0065] 3) FC>2.5, P<0.05, combined with Gene Ontology functional enrichment analysis (Gene Ontology functional enrichment analysis) and Kyoto Encyclopedia of Genes and Genomes pathway analysis (Kyoto Encyclopedia of Genes and Genomes pathway analysis) and breast cancer related literature After searching, ...

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Abstract

The invention provides a marker for breast cancer diagnosis and a screening method thereof, belonging to the field of biotechnology. By screening tissue specimens of breast cancer patients, a tRF&tiRNA marker for breast cancer diagnosis is obtained: 5′-tiRNA Val , 5'-tiRNA provided by the invention Val It is a low-expressed tiRNA in breast cancer, which is closely related to the proliferation and metastasis of breast cancer, and has potential value in early diagnosis and prognosis judgment.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a marker for breast cancer diagnosis and a screening method thereof. Background technique [0002] Breast cancer ranks first in incidence and sixth in mortality among female malignant tumors in my country, seriously endangering people's health and life. Despite great breakthroughs in drug sensitivity and surgical techniques, the frequent occurrence of recurrence and metastasis is still the main cause of death in patients with advanced breast cancer. The current clinical methods for diagnosing breast cancer mainly rely on physical examination and imaging examination, but these methods have low sensitivity for the detection of early breast cancer, and serum markers commonly used to diagnose breast cancer are both sensitive and specific for early diagnosis. not ideal. [0003] Therefore, it is of great significance to deeply understand the occurrence and development mechan...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/178C12Q2600/158C12Q2600/118
Inventor 严枫
Owner JIANGSU CANCER HOSPITAL