Immunosensor for detecting ZEN (zearalenone) and preparation method thereof

Abstract

The invention discloses an immunosensor for detecting ZEN (zearalenone). The immunosensor is prepared by a method with the following steps of (1) pretreatment of a glassy carbon electrode: polishing the glassy carbon electrode by aluminum oxide powder into a mirror surface, sequentially cleaning in anhydrous ethyl alcohol and acetone by ultrasonic waves, and airing; (2) treatment of the glassy carbon electrode: dripping dispersion liquid of molybdenum disulfide and thionine compound onto the glassy carbon electrode, and airing; then, dripping a platinum nanoparticle-modified ZEN monoclonal antibody solution, and airing; soaking in a 0.5% to 10% bovine serum albumin solution, and sealing, so as to obtain the required immunosensor. The immunosensor has the advantages that the sensitivity andrapidity effects are realized, and the application range is broad; the immunosensor is suitable for quickly detecting ZEN in biological samples of blood, urine and the like, and can be applied to hospitals and physical examination organizations, so as to provide quick and reliable basis for the judging of ZEN-related diseases.

Description

technical field [0001] The invention relates to the technical field of testing or analyzing material safety, in particular to an immunosensor for detecting zearalenone and a preparation method thereof. Background technique [0002] Zearalenone (ZEN) is a dihydroxybenzoic acid lactone metabolite with estrogen activity produced by a variety of Fusarium species, which is widely found in corn, wheat, sorghum, rice and other cereals. The structure of ZEN is similar to that of endogenous estrogen, and it can specifically bind to estrogen receptors, thereby inducing a series of reproductive toxicity and teratogenic effects; it can also cause lipid peroxidation in the body, causing damage to the liver and kidneys; In addition, ZEN also has certain toxic effects such as carcinogenicity, immunotoxicity, and teratogenicity. ZEN is stable in nature and is not easy to decompose during food storage, processing and cooking, so it is easy to remain in various processed raw materials. After...

AI Technical Summary

Problems solved by technology

Through the preliminary investigation and research, it was found that 3 parts of the blood of 260 normal people contained ZEN, and 18 parts of their urine contained ZEN, which caused huge potential risks to human health.

[0003] At present, there are mainly two methods for risk assessment of ZEN: one is to measure the content of ZEN in cereals and other foods and combine consumer dietary surveys to obtain the exposure of human pollutants. This assessment method is simple, but due to the variety of sources of ZEN, Including different food, air, water, skin contact, etc., so this method is generally not accurate and reliable; the second is to measure the content of ZEN in blood and urine, and calculate the exposure of pollutants through the human metabolic coefficient

The second method is accurate and reliable, but because the content of ZEN in blood and urine is very low, generally at the ng level, the accuracy, sensitivity and reliability of the detection technology become the bottleneck of this risk assessment method

Thin-layer chromatography is low in cost and easy to operate, but semi-quantitative visual inspection is greatly affected by subjectivity; ELISA has strong specificity, simple sample pretreatment, and short analysis time, but the enzyme activity is easily affected by operating conditions and prone to occurrence False positive; high performance liquid chromatography and mass spectrometry have good sensitivity, accuracy and stability, and are suitable for various samples containing complex components. However, this method is only common in scientific research institutes and testing institutions, and is not suitable for grassroots application and promotion, and requires complex pre-processing methods to ensure the accuracy of results, and cannot achieve rapid detection and on-site instant analysis

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