A kind of antibacterial fusion peptide and its preparation method and application

Abstract

The invention discloses an antimicrobial fusion peptide. The peptide chain sequence of the antimicrobial fusion peptide is SISLLYGRKKRRQRRR. The invention further provides another antimicrobial fusionpeptide of which the peptide chain sequence is YGRKKRRQRRRSISLL. The invention provides a preparation method of the antibacterial fusion peptide. The preparation method comprises the following steps:coupling corresponding amino acids from the carbon terminal to the nitrogen terminal according to the sequence of a target antibacterial fusion peptide; washing and blowing deprotection groups to dryness after coupling of the nitrogen-terminated amino acid is completed; cutting, carrying out suction filtration and fully washing and centrifuging by using ice diethyl ether to remove supernatant forthree times or above; dissolving the dried solid powder by using deionized water, separating, purifying, collecting main peak effluent and carrying out freeze drying to obtain the target antimicrobial fusion peptide. The invention further discloses application of the antimicrobial fusion peptide in medicines. According to the preparation method disclosed by the invention, the fusion peptide withan antibacterial effect is obtained on the basis of anticancer peptide; in addition, the antibacterial effect of the antimicrobial fusion peptide is close to the antibacterial effect of a powerful antimicrobial drug kanamycin, so that a basis is provided for further research and development of drugs with dual effects of resisting cancer and bacteria.

Description

technical field

[0001] The invention relates to the technical field of antibacterial drugs, in particular to an antibacterial fusion peptide. Background technique

[0002] There are three sources of existing antimicrobial peptides, natural product extraction, chemical synthesis and biological synthesis.

[0003] About chemical synthesis:

[0004] Amino acids are amphoteric substances, generally containing amino groups (-NH 2 ) and carboxyl (-COOH) are the basic raw materials for chemical synthesis of antimicrobial peptides. Researchers usually use amino groups that do not participate in the condensation reaction (-NH 2 ), carboxyl (-COOH) and side chains with active groups are protected, and after all amino acids are coupled according to the antimicrobial peptide sequence, the deprotection reaction is carried out.

[0005] Cancer is a disease that damages health and is life-threatening due to the excessive proliferation and proliferation of abnormal cells in the body. A...

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