Mesenchymal stem cell injection for treating cerebral ischemic stroke and preparation method

A technology of ischemic stroke and mesenchymal stem cells, applied in the field of cell biology, can solve the problems of prolonged hospitalization and huge long-term medical costs, and achieve the effects of improving acute inflammation, high vitality, and ensuring safety

Inactive Publication Date: 2018-10-09
北京山龟干细胞生物科技有限公司
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

The long-term medical costs of stroke are substantial, with many patients requiring prolonged hospitali

Method used

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  • Mesenchymal stem cell injection for treating cerebral ischemic stroke and preparation method
  • Mesenchymal stem cell injection for treating cerebral ischemic stroke and preparation method

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Embodiment Construction

[0042] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments, so that those skilled in the art can implement it with reference to the description.

[0043] It should be understood that terms such as "having", "comprising" and "including" used herein do not exclude the presence or addition of one or more other elements or combinations thereof.

[0044] 1. Preparation of umbilical cord mesenchymal stem cells

[0045] Operate in an ultra-clean workbench, a), umbilical cord separation: take a fresh full-term healthy fetal umbilical cord, wash it with normal saline containing 2 times 100U / mL penicillin and streptomycin to remove blood stains, peel off Walton's jelly, peel off the After the Walton's glue is cut to less than 1mm 3 small pieces;

[0046] b) Primary culture of mesenchymal cells: Evenly spread the shredded Walton's jelly on the bottom of the cell culture vessel, slowly add 10 mL of serum-free medi...

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Abstract

The invention discloses mesenchymal stem cell injection for treating cerebral ischemic stroke and a preparation method. The injection is prepared from activated mesenchymal stem cells with the quantity of 5*10<5> to 2*10<6>/mL, human serum albumin with the mass/volume ratio of 1 percent to 5 percent, mannitol injection with the mass/volume ratio of 1 percent to 8 percent, compound vitamin injection with the mass/volume ratio of 1 percent to 5 percent, 50 percent glucose injection with the mass/volume ratio of 1 percent to 5 percent and the balance of normal saline. The mesenchymal stem cell injection for treating the cerebral ischemic stroke, disclosed by the invention, releases pro-inflammatory factors including TNF (Tumor Necrosis Factor)-alpha, IL (Interleukin)-1, IL-6 and the like in an inflammatory damage process after cerebral ischemia; the mesenchymal stem cell injection is injected to treat the cerebral ischemic stroke.

Description

technical field [0001] The invention relates to the technical field of cell biology, more specifically, the invention relates to a mesenchymal stem cell injection for treating ischemic stroke and a preparation method thereof. Background technique [0002] Ischemic stroke is the most common type of stroke. It is due to the blockage of blood flow in the brain, and the lack of blood and oxygen in the brain causes nerve damage, which can lead to long-term or permanent disability. The current treatment options for ischemic stroke are very limited. The only options include thrombolytic agents such as tPA, or surgical mechanical thrombectomy and reperfusion surgery, and these methods are best limited to a few hours after the onset of stroke (3-6h ) within the implementation, it is possible to obtain curative effect. Due to various reasons, many patients often miss this extremely short optimal treatment time window, and finally have no choice but to simply accept supportive or pall...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61K35/51A61K9/08A61P9/10A61P29/00C12N5/0775
CPCA61K35/28A61K9/0019A61K9/08A61K35/51A61P9/10A61P29/00C12N5/0662C12N5/0663C12N5/0667C12N5/0668C12N2501/2301C12N2501/25
Inventor 郝好杰徐瑛李梓源陈惠华周严恒易军
Owner 北京山龟干细胞生物科技有限公司
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