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A method and system for image reconstruction

A technology for image reconstruction and original image, applied in the field of super-resolution microscope image system and reconstruction, which can solve problems such as limiting the duration of imaging

Active Publication Date: 2022-05-24
GUANGZHOU COMPUTATIONAL SUPER RESOLUTION BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Simply increasing illumination power beyond the threshold induces nonlinear photobleaching of EGFP, which limits imaging duration

Method used

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  • A method and system for image reconstruction
  • A method and system for image reconstruction
  • A method and system for image reconstruction

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Embodiment Construction

[0054] In order to illustrate the technical solutions of the embodiments of the present application more clearly, the following briefly introduces the accompanying drawings used in the description of the embodiments. Obviously, the accompanying drawings in the following description are only some examples or embodiments of the present application. For those skilled in the art, the present application can also be applied to the present application according to these drawings without any creative effort. other similar situations. Unless obvious from the locale or otherwise specified, the same reference numbers in the figures represent the same structure or operation.

[0055] As shown in this application and in the claims, unless the context clearly dictates otherwise, the words "a", "an", "an" and / or "the" are not intended to be specific in the singular and may include the plural. Generally speaking, the terms "comprising" and "comprising" only imply that the clearly identified...

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Abstract

An image reconstruction method, the method includes: obtaining super-resolution images based on at least two groups of original images. Wherein, each group of original images includes at least two original images, and at least one group of original images in the at least two groups of original images has one or more original images identical to other groups of original images in the at least two groups of original images.

Description

technical field [0001] The invention relates to a super-resolution microscope, in particular to a system and a reconstruction method of a super-resolution microscope image. Background technique [0002] With the development of modern biomedicine, Super-Resolution (SR) microscopy has become an increasingly important research tool. In order to obtain the required spatial resolution images, conventional super-resolution microscopy is often achieved by increasing the amount of illumination. However, increasing the amount of illumination (eg, increasing illumination time, increasing illumination power) can lead to severe photobleaching and phototoxicity. Then, the application of super-resolution microscopy to living cells is greatly limited by photobleaching and phototoxicity. In recent years, Structured Light Illumination Microscopy (SIM) has excelled in achieving high spatial resolution with a small number of photons, with higher photon efficiency. Studies have shown that SI...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G06T3/40G06T5/00
CPCG06T3/4053G06T5/70
Inventor 黄小帅
Owner GUANGZHOU COMPUTATIONAL SUPER RESOLUTION BIOTECH CO LTD