Embedding method of fiber-shaped sample for axial ultrathin slice

Abstract

The invention discloses an embedding method of a fiber-shaped sample for an axial ultrathin slice. The embedding method comprises the following steps: fixing the fiber-shaped sample on a substrate toenable the fiber-shaped sample to be linear on the substrate; adding a liquid embedding agent into an open container; then covering the substrate fixed with the fiber-shaped sample on the top of the open container and enabling the surface fixed with the fiber-shaped sample of the substrate to be arranged toward the open container; fixing the substrate and the open container; then integrally overturning the substrate and the open container; reversely buckling the open container on the surface fixed with the fiber-shaped sample of the substrate; curing the liquid embedding agent to form an embedding block; separating the open container and the substrate and enabling the fiber-shaped sample to be located at a position of the topmost end of the embedding block, so as to obtain an embedded fiber-shaped sample for the axial ultrathin slice. The method disclosed by the invention can be used for accurately determining the position of the sample and remarkably improving the later-period block trimming efficiency and the success rate of slicing.

Description

technical field [0001] The present application relates to the technical field of ultrathin sections, in particular, to an embedding method for fibrous samples used in axial ultrathin sections. Background technique [0002] In traditional methods for axial ultrathin sectioning of fibrous samples including fibers, the fibrous sample is usually placed in an embedding plate, then poured into a resin for embedding, and the resulting embedding block is subjected to ultrathin sectioning deal with. However, this method is usually difficult to ensure that the fibrous sample is directly exposed to the end of the embedding block to be cut. In the subsequent trimming process, it is generally difficult to accurately locate the embedded fibrous material under a microscope, which may cause miscutting, which in turn leads to the failure of the fibrous sample to be characterized when observing the section with a transmission electron microscope (TEM). This will greatly waste the time and e...

AI Technical Summary

Problems solved by technology

This embedding method overcomes the problem that the fiber cannot be accurately positioned when the ultra-thin section is trimmed after the fiber is embedded with epoxy resin in the early stage, and greatly improves the efficiency of later trimming and the success rate of slicing.

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