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Method for determining n-nitrosamines peculiar to magnetic graphene and tobacco and sample pretreatment method

A technology of sample pretreatment and magnetic graphene, applied in the field of tobacco chemical inspection, can solve the problems of complicated operation process and low purification efficiency, and achieve the effects of simple and fast operation, high purification efficiency and improved purification efficiency.

Inactive Publication Date: 2018-10-30
CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The purpose of the present invention is to provide a sample pretreatment method for the determination of tobacco-specific N-nitrosamines, thereby solving the problems of complicated operation process and low purification efficiency in the existing sample processing methods

Method used

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  • Method for determining n-nitrosamines peculiar to magnetic graphene and tobacco and sample pretreatment method
  • Method for determining n-nitrosamines peculiar to magnetic graphene and tobacco and sample pretreatment method
  • Method for determining n-nitrosamines peculiar to magnetic graphene and tobacco and sample pretreatment method

Examples

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Embodiment 1

[0054] The extraction method of tobacco-specific N-nitrosamines in cigarette mainstream smoke, tobacco and tobacco products of this embodiment adopts the following steps to prepare the sample solution to be tested: add the total particulate matter in Kentucky 3R4F reference cigarette mainstream smoke (puffing 5 cigarettes) was added 15mL of 0.1mol / L ammonium acetate solution, then ultrasonically extracted for 30min, and the supernatant was taken as the sample extract.

[0055] Add 0.5mL of magnetic graphene dispersion liquid (concentration in terms of graphene is 20mg / mL) to 10mL sample extract, discard the supernatant under the action of magnet after vortex 10min, add 2.0mL acetone desorption 5.0min, obtain Sample solution to be tested.

[0056] The magnetic graphene dispersion is composed of 80nm Fe 3 o 4 Particles and graphene are prepared by vortex mixing at 1000 rpm for 3 minutes at a mass ratio of 1:1.

Embodiment 2-3

[0058] The extraction method of tobacco-specific N-nitrosamines in cigarette mainstream smoke, tobacco and tobacco products in Example 2-3 is basically the same as Example 1, the only difference being that Example 2 uses aminated graphene, and Example 3 Use carboxylated graphene.

Embodiment 4

[0060] The method for extracting tobacco-specific N-nitrosamines in cigarette mainstream smoke, tobacco, and tobacco products of this embodiment refers to the method in Example 1 to prepare a sample extract, and then adds NaCl (control concentration is 20mmol L) to the sample extract. -1 ), add magnetic graphene dispersion 0.5mL (concentration in terms of graphene is 20mg / mL), vortex 20min and discard the supernatant under the action of a magnet, add 1.0mL of acetonitrile for desorption for 5.0min, and obtain the sample to be tested solution.

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Abstract

The invention relates to a method for determining n-nitrosamines peculiar to magnetic graphene and tobacco and a sample pretreatment method. The sample pretreatment method includes the steps of addingmagnetic graphene to sample extraction buffer for extraction; eluting target analytes absorbed to the magnetic graphene; collecting eluant which is a to-be-determined solution. With the method, specific adsorption of the target analytes is realized by the aid of multiple interactions of pi-pi, dewatering and the like which can occur between n-nitrosamines peculiar to magnetic graphene and tobacco, and removal of interferences from complex matrix and enrichment of the target analytes are realized; the determination result shows that no interfering substances affect the quantification of the target analytes, the treatment method of the sample extract has good purification capability for actual samples, and purification efficiency is improved.

Description

technical field [0001] The invention belongs to the technical field of tobacco chemical testing, and in particular relates to a method for measuring magnetic graphene, tobacco-specific N-nitrosamines, and a sample pretreatment method. Background technique [0002] Tobacco-specific N-nitrosamine compounds are not only important compounds in the Hoffman list and the "List of Harmful and Potentially Harmful Substances in Tobacco Products and Smoke" issued by the US Food and Drug Administration, but also included in the "Smoke-free Smoke-free Substances List" of the International Agency for Research on Cancer. Important ingredients in the list of 28 harmful substances in tobacco products. For example, N-nitrosonornicotine (NNN) and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) are recognized as Class I by the International Agency for Research on Cancer carcinogen. Therefore, it is of great significance to accurately analyze the content of tobacco-specific N-nitrosamines...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88
Inventor 罗彦波陈小静庞永强朱风鹏姜兴益张洪非李翔宇
Owner CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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