Method for culturing in-vitro fertilization embryo of oocyte collected from living cow or in vitro

An embryo culture and oocyte technology, applied in cell dissociation methods, cell culture active agents, germ cells, etc., can solve the problems of limited wide application, low efficiency, and high cost of in vitro fertilization embryo production
CN108728404AActive Publication Date: 2018-11-02天津力牧生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
天津力牧生物科技有限公司
Publication Date
2018-11-02
Patent Text Reader

Abstract

The invention relates to a method for culturing an in-vitro fertilization embryo of an oocyte collected from a living cow or in vitro. The method comprises the following steps: collection and in-vitromaturation of the oocyte, wherein the oocyte can be collected in vitro or from the living cow; in-vitro fertilization, wherein mature COCs is cultured in a fertilization culture solution and then themature COCs and sperm suspension are subjected to co-incubation culture; and in-vitro culture of the embryo and cryopreservation in liquid nitrogen. The method has the excellent technical effect as shown in specification.
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Description

technical field

[0001] The invention belongs to the technical field of animal reproduction, and relates to a technology for agricultural-animal husbandry and veterinary reproduction, in particular to a method for culturing bovine in vitro fertilized embryos. In addition, the present invention also relates to the use of related culture fluids for the cultivation of bovine in vitro fertilized embryos. Application in in vitro fertilization embryo culture. Further, the present invention also relates to a method for culturing bovine IVF embryos using the relevant culture fluid for culturing bovine IVF embryos. In particular, the method for culturing bovine IVF embryos of the present invention has excellent technical effects. In particular, the present invention relates to a method for in vitro fertilization and embryo culture of live or isolated bovine oocytes. Background technique

[0002] In Vitro Fertilization (In Vitro Fertilization) or (external fertilization) refers to th...

Claims

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