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A kind of kiwifruit seed initiator and initiating method

A technology of kiwifruit and initiator, which is applied in the fields of seed and rhizome treatment, botanical equipment and methods, biocides, etc., can solve the problems of undiscovered kiwifruit seed initiator and initiation method, unfavorable production cost, long treatment time, etc., and achieve Improve the germination rate and germination uniformity, save production costs, and the effect of simple method

Active Publication Date: 2021-05-25
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is cumbersome and takes a long time to process. It usually takes more than two months, and the seedling rate is low. In production practice, it is not conducive to saving production costs.
Retrieval of relevant prior art has not found any reports on kiwi fruit seed initiator and initiation method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The original source of Aspergillus niger used in this example was purchased from the standard strain of the China General Microorganism Culture Collection and Management Center, and its preservation number is CGMCC 3.3928, which meets the National Military Standard GJB 150.10A-2009 of the People's Republic of China and the National Military Standard of the People's Republic of China GJB 4.10-83.

[0028] All other reagents used were of analytical grade.

[0029] The priming solution for kiwifruit seeds was prepared as follows:

[0030] 1. Activate Aspergillus niger with the preservation number CGMCC 3.3928, inoculate it on a PDA plate, and culture it at a constant temperature of 28±2° C. for 3 days to obtain activated Aspergillus niger. Store the strains at 4°C for future use.

[0031] 2. Preparation of green tea water extract: mix fresh green tea with 95°C water according to the material-to-liquid ratio of 1:50 (g / mL), extract for 10 minutes, cool to room temperature...

Embodiment 2

[0042] The original source of Aspergillus niger used in this example was purchased from the standard strain of the China General Microorganism Culture Collection and Management Center, and its preservation number is CGMCC 3.3928, which meets the National Military Standard GJB 150.10A-2009 of the People's Republic of China and the National Military Standard of the People's Republic of China GJB 4.10-83.

[0043] All other reagents used were of analytical grade.

[0044] The priming solution for kiwifruit seeds was prepared as follows:

[0045] 1. Activate Aspergillus niger with the preservation number CGMCC 3.3928, inoculate it on a PDA plate, and culture it at a constant temperature of 28±2° C. for 5 days to obtain activated Aspergillus niger. Store the strains at 4°C for future use.

[0046] 2. Preparation of green tea water extract: mix green tea with water at 100°C according to the material-to-liquid ratio of 1:100 (g / mL), extract for 20 minutes, cool to room temperature,...

Embodiment 3

[0054] The original source of Aspergillus niger used in this example was purchased from the standard strain of the China General Microorganism Culture Collection and Management Center, and its preservation number is CGMCC 3.3928, which meets the National Military Standard GJB 150.10A-2009 of the People's Republic of China and the National Military Standard of the People's Republic of China GJB 4.10-83.

[0055] All other reagents used were of analytical grade.

[0056] The priming solution for kiwifruit seeds was prepared as follows:

[0057] 1. Activate Aspergillus niger with the preservation number CGMCC 3.3928, inoculate it on a PDA plate, and culture it at a constant temperature of 28±2° C. for 1 day to obtain activated Aspergillus niger. Store the strains at 4°C for future use.

[0058] 2. Preparation of green tea water extract: mix green tea powder with water at 80°C according to the ratio of material to liquid 1:20 (g / mL), extract for 60 minutes, cool to room temperat...

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PUM

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Abstract

The invention provides a kiwifruit seed initiator and an initiator method, belonging to the technical field of seedling cultivation. The kiwifruit seed initiator proposed by the invention is the water extract of green tea containing Aspergillus niger spores. The main steps of the priming method include: under the light condition of 10±2°C, using the green tea water extract containing Aspergillus niger spores as the priming agent for 24-150 hours, and alternately culturing the priming-treated kiwifruit seeds in the dark and in the light. The initiator of the invention has low cost and good initiating effect without adding any additives. At the same time, the invention also significantly improves the germination rate and germination uniformity of kiwifruit seeds, and the method is simple, environmentally friendly and safe, and provides technical support for seedling cultivation.

Description

technical field [0001] The invention relates to the technical field of seedling cultivation, in particular to a kiwifruit seed initiator and an initiator method. Background technique [0002] Kiwifruit ( kiwifruit ) is a dioecious perennial wild fruit tree mainly produced in my country. It has become an important emerging fruit in the world because of its unique edible value and medicinal value. It has been introduced and cultivated in many countries such as the United States, Italy, Australia, Chile, France, Greece, Iran, Israel, New Zealand, Portugal, Spain, Turkey and Switzerland. [0003] The seed priming technology is proposed based on the biological mechanism of seed germination. The purpose is to promote seed germination, improve the stability and uniformity of germination time, reduce the standard deviation of germination time, and improve the resistance and stability of seedlings. quality and improve nutritional status. Initiation is mainly achieved through osmoti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01C1/00A01N65/08A01N63/30A01P21/00
CPCA01C1/00A01N65/00A01N65/08A01N63/30
Inventor 刘学诗石怡宋芹张楠朱红桥
Owner ANHUI AGRICULTURAL UNIVERSITY