Method for producing high-content salinomycin premix

Abstract

The invention discloses a method for producing a high-content salinomycin premix. The method comprises adding a flocculant to flocculate a salinomycin fermentation liquid, carrying out standing, removing the supernatant, collecting the floc, adding auxiliary materials into the floc, and carrying out drying and crushing to obtain salinomycin premix particles with salinomycin content of 50-80%. Through experiments, the type and content of the flocculant in the premix are found and the biological efficacy of the salinomycin premix is significantly affected. Nonionic polyacrylamide as a flocculantis added into the fermentation liquid at a concentration of 3.50 mg / mL. Through use of the bran excipients, the premix with 65% of salinomycin is prepared. The premix is used for the culture of ducklings and realizes the survival rate of 99% and daily gain of 71 g / d.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a production method of a salinomycin premix, more specifically to a production method of a high-content salinomycin premix. Background technique [0002] Salinomycin belongs to polyether monocarboxylic acid antibiotics, which is produced by fermentation of Streptomyces albicans. It has a special ring structure and is a typical ionophore antibiotic. Through the enhancement of the permeability of the lipid barrier on the membrane, the ion concentration inside and outside the cell is changed, the osmotic pressure is affected, and the cell is finally disintegrated. It has a strong inhibitory and killing effect on most Gram-positive bacteria and various coccidia. It is not easy to produce drug resistance and cross-resistance, it is excreted rapidly and the residual amount is extremely low. It can be used as an anti-coccidial agent for chickens and a growth promoter for piglets and f...

AI Technical Summary

Problems solved by technology

During the experiment, the addition of calcium carbonate was reduced by prolonging the fermentation cycle, and a chlortetracycline premix with a chlortetracycline content of 23.3% was prepared. However, in this invention, the requirements for the cultivation of Streptomyces aureus were high, and the final premix The content of aureomycin in the product is still low

[0006] Chinese patent publication CN 101884621A discloses a preparation method of a salinomycin granule premix: adjust the pH of the salinomycin fermentation broth with a potency of 60,000-100,000 μ / mL to 6.5-10.0 with alkali to obtain an alkaline fermentation broth; Calcium carbonate equivalent to 0-25% of the mass of the fermentation broth is added to obtain a salinomycin feed liquid; the feed liquid is spray-dried to obtain a salinomycin granule premix, and the salinomycin premix prepared by this method , although it has high stability, the content of salinomycin is only 31%, and there is still the problem of adding a lot of excipients

[0007] To sum up, the content of salinomycin in the products obtained by the preparation method in the prior art is still at a relatively low level, resulting in that most of the materials actually circulated and transported are auxiliary materials,

[0008] In a disguised form, the production and circulation costs of the active ingredient salinomycin are increased

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