Extraction method of aloe emodin

A technique for extracting aloe-emodin and its extraction method, which is applied in the field of extracting aloe-emodin, can solve the problems of reducing the biological activity of aloe-emodin, and achieve the effects of easy industrialization, less by-products, and simple operation process

Inactive Publication Date: 2018-11-06
金华市景和科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Aloe-emodin belongs to anthraquinone compounds containing phenolic hydroxyl groups, and it is easy to decompose with the increase of temperature and the extension of extraction time. The traditional heat extraction and solvent extraction methods have seriously reduced the biological activity of aloe-emodin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A method for extracting aloe-emodin, comprising: pretreatment of aloe-emodin, preparation of aloe-emodin crude extract and purification of the crude extract, specifically comprising the following steps:

[0020] 1) Aloe skin pretreatment: clean the aloe skin, vacuum freeze-dry, crush, pass through a 60-mesh sieve, then add 5 times the weight of the aloe skin in deionized water containing lactitol and glycyrrhizic acid to soak for 30 minutes, filter and collect the filter residue, and stored at low temperature for later use;

[0021] 2) Preparation of aloe-emodin crude extract: first add a mixture of glacial acetic acid and uridine triphosphate to aloe residue, stir at 200 rpm for 10 minutes, then add ethanol 8 times the weight of aloe residue, and extract under microwave radiation After 2 hours, filter while hot, wash the filter paper 3 times with ethanol, combine the filtrates, concentrate by rotary evaporation under reduced pressure, and freeze-dry to obtain the crude...

Embodiment 2

[0030] A method for extracting aloe-emodin, comprising: pretreatment of aloe-emodin, preparation of aloe-emodin crude extract and purification of the crude extract, specifically comprising the following steps:

[0031] 1) Aloe skin pretreatment: clean the aloe skin, vacuum freeze-dry, crush, pass through a 60-mesh sieve, then add 7 times the weight of the aloe skin in deionized water containing lactitol and glycyrrhizic acid to soak for 40 minutes, collect the filter residue after filtration, and stored at low temperature for later use;

[0032] 2) Preparation of aloe-emodin crude extract: first add a mixture of glacial acetic acid and uridine triphosphate to aloe residue, stir at 300rpm for 10 minutes, then add ethanol 10 times the weight of aloe residue, and extract under microwave radiation After 3 hours, filter while hot, wash the filter paper 4 times with ethanol, combine the filtrates, concentrate by rotary evaporation under reduced pressure, and freeze-dry to obtain the...

Embodiment 3

[0041] A method for extracting aloe-emodin, comprising: pretreatment of aloe-emodin, preparation of aloe-emodin crude extract and purification of the crude extract, specifically comprising the following steps:

[0042] 1) Aloe skin pretreatment: clean the aloe skin, vacuum freeze-dry, crush, pass through a 60-mesh sieve, then add 6 times the weight of the aloe skin in deionized water containing lactitol and glycyrrhizic acid and soak for 30 minutes, filter and collect the filter residue, and stored at low temperature for later use;

[0043]2) Preparation of aloe-emodin crude extract: first add a mixture of glacial acetic acid and uridine triphosphate to aloe residue, stir at 400rpm for 20min, then add ethanol 8 times the weight of aloe residue, and extract under microwave radiation After 2.5 hours, filter while hot, wash the filter paper 3 times with ethanol, combine the filtrates, concentrate by rotary evaporation under reduced pressure, and freeze-dry to obtain the crude alo...

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PUM

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Abstract

The invention discloses an extraction method of aloe emodin. The specific preparation method comprises the steps as follows: aloe peel is cleaned, dried, crushed and sieved, then, the obtained material is soaked in deionized water containing lactitol and glycyrrhizic acid, aloe filter residues are collected, then, a mixture of glacial acetic acid and uridine triphosphate is added to the aloe filter residues, the mixture is uniformly stirred, ethanol is added, extraction is performed under assistance of microwave irradiation, the filtration is performed immediately, filter paper is washed withethanol, filtrates are mixed, freeze drying is performed after rotary evaporation under reduced pressure, a crude extract of aloe emodin is obtained, finally, the crude extract of aloe emodin is purified with a SephadexLH-20 gel column, and the aloe emodin is obtained. The extraction method is convenient to operate, energy-saving, efficient and high in extraction rate, few in byproduct and facilitates industrial promotion. The extracted aloe emodin has high purity and good bioactivity and can be widely applied to fields of preparation of healthcare products, beautifying and skin care and medicine intermediates.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine, in particular to an extraction method of aloe-emodin. technical background [0002] Aloe is a perennial evergreen herb of the Liliaceae Aloe family, commonly known as "old aloe". It is native to tropical arid regions of Africa and is distributed almost all over the world. Since the 1990s, the cultivation and application development of aloe has received widespread attention at home and abroad. At present, it is widely planted in southern my country. It is a plant with high medicinal value. It tastes bitter and cold, and returns to the liver, stomach, and large intestine meridian. It has the effects of purging heat, guiding accumulation, killing insects, and cooling the liver. In recent years, with the in-depth research on aloe plants, the application value of aloe has gradually been recognized by people. Using modern scientific means to extract and separate the main active ...

Claims

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Application Information

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IPC IPC(8): C07C46/10C07C50/34
CPCC07C46/10C07C2603/24C07C50/34
Inventor汪珂
Owner金华市景和科技有限公司