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A preparation process of spirulina protein and its neuroprotective application

A neuroprotective and spirulina technology, applied in the field of marine biology, can solve problems such as no reports on the application of spirulina protein, and achieve the effect of alleviating the toxicity of nerve damage

Active Publication Date: 2021-04-30
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] After searching, there is no report on the application of spirulina protein in the field of neurodegenerative diseases

Method used

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  • A preparation process of spirulina protein and its neuroprotective application
  • A preparation process of spirulina protein and its neuroprotective application
  • A preparation process of spirulina protein and its neuroprotective application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The preparation of embodiment 1 spirulina protein

[0040] S1: Take spirulina powder, add Tris-HCl buffer solution (pH=7.4) to prepare a 20% suspension, soak for 1 day, then freeze at -20°C, thaw at 4°C, and freeze-thaw repeatedly 3 times , centrifuge at a speed of 5000rpm / min for 30min, separate the supernatant, which is the crude protein extract;

[0041]S2: Slowly add ammonium sulfate to the crude protein extract described in step S1 to 50% saturation while stirring, let stand overnight at 4°C, centrifuge at 5000rpm / min for 30min, and separate the precipitate;

[0042] S3: Take the precipitate described in step S2, redissolve it with primary water, dialyze in a 3.5KDa dialysis bag to remove salt, first dialyze with running water for 3 days, then dialyze with deionized water for 3 times, and change it every 12 hours Deionized water, take the dialyzed protein solution, freeze-dry to obtain spirulina protein, and store it at -20°C for later use.

Embodiment 2

[0043] The preparation of embodiment 2 spirulina protein

[0044] S1: Take spirulina powder, add Tris-HCl buffer (pH=7.4) to prepare a 20% suspension, soak for 2 days, then freeze at -30°C, thaw at 4°C, and freeze and thaw repeatedly 4 times , centrifuge at a speed of 6000rpm / min for 30min, separate the supernatant, which is the crude protein extract;

[0045] S2: Slowly add ammonium sulfate to the crude protein extract described in step S1 to 50% saturation while stirring, let stand overnight at 4°C, centrifuge at 6000rpm / min for 30min, and separate to obtain a precipitate;

[0046] S3: Take the precipitate described in step S2, redissolve it with primary water, dialyze in a 3.5KDa dialysis bag to remove salt, first dialyze with running water for 3 days, then dialyze with deionized water for 4 times, and change it every 12 hours Deionized water, take the dialyzed protein solution, freeze-dry to obtain spirulina protein, and store it at -20°C for later use.

Embodiment 3

[0047] The preparation of embodiment 3 spirulina protein

[0048] S1: Take spirulina powder, add Tris-HCl buffer (pH=7.4) to make a 20% suspension, soak for 1 day, then freeze at -40°C, thaw at 4°C, and freeze-thaw repeatedly 5 times , centrifuge at a speed of 6000rpm / min for 40min, separate the supernatant, which is the crude protein extract;

[0049] S2: Slowly add ammonium sulfate to the crude protein extract described in step S1 to 50% saturation while stirring, let stand overnight at 4°C, centrifuge at 6000rpm / min for 40min, and separate to obtain a precipitate;

[0050] S3: Take the precipitate described in step S2, redissolve it with primary water, dialyze in a 3.5KDa dialysis bag to remove salt, first dialyze with running water for 3 days, then dialyze with deionized water for 5 times, and change it every 12 hours Deionized water, take the dialyzed protein solution, freeze-dry to obtain spirulina protein, and store it at -20°C for later use.

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Abstract

The invention belongs to the technical field of marine organisms, and relates to a preparation process of spirulina protein and its neuroprotective application. Specifically, the invention discloses a preparation method of spirulina protein. The spirulina powder is soaked, freeze-thawed, salted out, dialyzed and freeze-dried to obtain the spirulina protein. The spirulina protein provided by the present invention can reduce the survival rate of the nerve cell strain PC-12 under oxidative damage, reduce the aggregation of the toxic protein α-synuclein of the Parkinson's disease model nematode strain NL5901, and inhibit the Huntington's disease model Caenorhabditis elegans (AM141 ) can alleviate the neurotoxicity caused by Aβ toxic protein in the Alzheimer's disease model Caenorhabditis elegans (CL2355), and has neuroprotective function.

Description

technical field [0001] The invention belongs to the technical field of marine organisms, and in particular relates to a preparation process of spirulina protein and its neuroprotective application. Background technique [0002] With the aging of the world population, there are more and more patients with neurodegenerative diseases closely related to aging. It is estimated that by 2050, the number of patients suffering from such diseases worldwide will reach 100 million. The pathogenesis and the search for effective prevention measures have become scientific research and social problems to be solved urgently. [0003] The pathogenesis of neurodegenerative diseases is very complicated, but researchers believe that although neurodegenerative diseases have different causes and have their own characteristics in the early stages of the disease, there are many similarities between different diseases, especially in Asian countries. At the cellular level, abnormal protein aggregatio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C07K1/30A61K38/16A23L33/185A61P25/28A61P25/16A61P25/14
CPCA23V2002/00A61K38/00A23L33/185A61P25/14A61P25/16A61P25/28C07K14/415A23V2200/322A23V2250/548
Inventor 胡丽莉王丁丁余程明许瑜玳潘子康杨雨澄
Owner GUANGDONG PHARMA UNIV
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