Preparation of fluorogold nano-clusters and application of fluorogold nano-clusters to tetracycline and copper fluorescent probes

Abstract

The invention discloses preparation of fluorogold nano-clusters and application of the fluorogold nano-clusters to tetracycline and copper fluorescent probes. The fluorogold nano-clusters (Au NCs) aresynthesized with a one-step method from casein separated from milk and carbon quantum dots as a stabilizer and a reducer for synthesis of the Au NCs. The Au NCs perform emission three times at 433 nm, 702 nm and 1052 nm under the excitation wavelength of 350 nm, tetracycline has a linear quenching effect at 702 nm, Cu<2+> is added to an Au NCs system after quenching, fluorescence recovers, and fluorescence intensity at 1052 nm is almost not affected in the overall process; tetracycline is added to the Au NCs, observation is carried out under a UV (ultraviolet) lamp with wavelength of 365 nm,red fluorescence of the Au NCs changes into yellow fluorescence, and after addition of Cu<2+>, the yellow fluorescence disappears gradually and the red fluorescence recovers gradually. Therefore, a near-infrared ratio fluorescence switch for tetracycline and Cu<2+> and UV visual dual-signal detection are established. The method has the advantages of adopting a fluorescence spectrum in a near infrared region and being small in interference, high in specificity and detection sensitivity, convenient to operate and capable of detecting tetracycline and Cu<2+> simultaneously.

Description

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AI Technical Summary

Benefits of technology

This patented technology allows us to create small particles that can be used to detect substances by measuring their ability to emit visible lights when excited or interacted with them. These tiny objects usually consist of two parts - one part contains atoms called molecules (moleculae), another containing smaller pieces made from these atom layers. When they hit each other, some energy will transfer between them through radiation exchange processes like electromagnetic waves. By analyzing this interaction's properties we get an understanding about how it works.

Problems solved by technology

This patents describes methods for creating stable metallon fluoroplasmoniums that can be excited by visible lights at different colors depending upon their structure. These structures include AuNPs made from various elements including carbons, nitrocellulose, chlorophyll, pyridylinediaminetranosulfonic acid, and proteins containing sulfur-based functionalities called thyrnolecs. Therapeutrons also known as diamond particles, consisting mainly of boron, tantaluminum, indanthrone, lutea flavonoid, rhodesia silver, and oxynaphthoquinones show promise applications in biological analysis technologies due to its ability to generate luminescences when exposed to certain types of energy sources. However, current techniques require expensive starting materials and complicated processes, making them difficult to use outside research settings worldwide.

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