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Method for detecting 8 kinds of mycotoxins of fructus citri medicae

A technology of mycotoxin and bergamot, which is applied in the field of traditional Chinese medicine detection, can solve the problems of susceptibility to fungal contamination, affecting the quality of Chinese medicinal materials and drug safety, and achieve the effect of high sensitivity and good detection limit

Inactive Publication Date: 2018-11-06
广东省药品检验所
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Problems solved by technology

[0003] Due to the hot and humid subtropical climate in Guangdong, Chinese herbal medicines are easily contaminated by fungi during the process of planting, collection, storage, and processing, resulting in the production of various mycotoxins, which seriously affect the quality and safety of Chinese herbal medicines.

Method used

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  • Method for detecting 8 kinds of mycotoxins of fructus citri medicae
  • Method for detecting 8 kinds of mycotoxins of fructus citri medicae
  • Method for detecting 8 kinds of mycotoxins of fructus citri medicae

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Embodiment 1

[0043] 1. Solution preparation:

[0044] 1) Preparation of mixed internal standard solution:

[0045] Precision pipetting 1.0mL 13 C 34 -FB1, 13 C 20 -OTA, 13 C 18 -ZEN standard solution in 10mL volumetric flask, except 13 C 34 -FB1 was diluted with methanol to the mark with acetonitrile, and mixed well. That is, the concentration is 2.55μg / mL 13 C 34 -FB1, at a concentration of 1.00 μg / mL 13 C 20 -OTA and a concentration of 2.51 μg / mL 13 C 18 -ZEN's standard internal standard intermediate solution. because 13 C 17 - The mother solution concentration of AFB1 standard solution is low (0.503μg / mL), which is used as a separate internal standard.

[0046] Precision pipetting 1.0mL 13 C 34 -FB1, 1.6mL 13 C 20 -OTA, 1.6mL 13 C 18 - ZEN standard internal standard intermediate solution, placed together in a 10ml volumetric flask, diluted with acetonitrile to the mark, that is, a solution containing 0.4μg / mL 13 C 34 -FB1, 0.1 μg / mL 13 C 20 -OTA, 0.4 μg / mL 13...

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Abstract

The invention discloses a method for measuring 8 kinds of mycotoxins of fructus citri medicae. According to the method, the 8 kinds of mycotoxins (ochratoxin A, zearalenone, fumonisin B1, sterigmatocystin, aflatoxin B1, aflatoxin B2, aflatoxin G1 and aflatoxin G2) are measured simultaneously in a system, a Thermo Hypersil GOLD chromatographic column is adopted for separation, and 0.1% formic acidwater-formic acid acetonitrile is used as a mobile phase; an electrospray ionization mode is adopted, and negative and positive ions scan simultaneously. A result shows that according to the method, the relations of the 8 kinds of mycotoxins in the linear range are good; the detection limit is at 0.16-5 ng / mL, the recovery rate at the low, medium and high adding levels is 79.0-112.8%, and an isotope internal standard method is adopted. According to the method, the matrix effect has little influence on the mycotoxin detection result, the accuracy is high, the precision degree is high, the specificity is high, and the method is simple, convenient and fast and can be used for multi-component measurement of mycotoxins in traditional Chinese medicine materials.

Description

technical field [0001] The invention relates to a method for determining eight kinds of mycotoxins in Guangfo hands, belonging to the field of traditional Chinese medicine detection. Background technique [0002] Bergamot is the ripe fruit of Rutaceae citrus (Citrus medica L.var.sarcodactylis Swingle), mainly produced in Guangdong, and also cultivated in Fujian, Sichuan, Zhejiang and other areas. Among them, the bergamot from Zhaoqing, Gaoyao, Deqing, Yunan and other places in Guangdong is called "Guangberg bergamot" and is an authentic product of Guangdong. Bergamot can not only be used as medicine, but also be used to make tea, brew wine, and even be made into delicacies. On May 31, 2016, the first batch of Guangdong legislation to protect Lingnan Chinese herbal medicines was born. Bergamot is one of the first eight kinds of Chinese herbal medicines selected. It can be seen that Guangbergmango, as an authentic medicinal material in Guangdong, has an important role in the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 胡佳哲赖宇红
Owner 广东省药品检验所
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