A method for detecting content of cyhalofop-methyl and saflufenacil in compound preparation

A technology of saflufenacil and cyhalofop-fop, which is applied in the field of analytical chemistry, can solve the problems of inseparability, waste of reagents, and long time consumption, and achieve the effects of high sensitivity, accurate detection results, and good reproducibility

Active Publication Date: 2021-05-18
SHANDONG WEIFANG RAINBOW CHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, no relevant patents have been found for the method of determining the content of cyhalofop-ethyl and saflufenacil together at home and abroad, while the detection method for the composition of cyhalofop-fop is a forward detection method of high performance liquid chromatography, and the detection method for the composition of saflufenacil is a high-efficiency Liquid chromatography reversed-phase detection method, and the currently disclosed method takes a long time to detect each component separately and wastes reagents, and the impurity peak cannot be separated from the active component peak by copying the detection method

Method used

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  • A method for detecting content of cyhalofop-methyl and saflufenacil in compound preparation
  • A method for detecting content of cyhalofop-methyl and saflufenacil in compound preparation
  • A method for detecting content of cyhalofop-methyl and saflufenacil in compound preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The chromatographic conditions for detection are as follows:

[0038] Variable wavelength ultraviolet detector, C18 chromatographic column 5.0μm250mm×4.6mm (I.D.);

[0039] Mobile phase: acetonitrile: water = 60: 40;

[0040] Detection wavelength: 232nm;

[0041] Flow rate: 1.0mL / min;

[0042] The specific detection method is:

[0043] Accurately weigh 0.05g of the standard cyhalofop-methyl and 0.0210g of saflufenacil in the same 50mL volumetric flask, dissolve and dilute to the mark with acetonitrile. Weigh about 0.8100g of a sample of 60g / L cyhalofop-methyl + 25g / L saflufenacil compound EC, place it in a 50mL volumetric flask, dissolve it with acetonitrile and dilute to the mark, and degas it by ultrasonic for 5 minutes. The standard sample was taken for liquid chromatography analysis, the injection volume was 5 μL, and the peak areas of cyhalofop-ethyl and saflufenacil were determined. Samples were taken for liquid chromatography analysis with an injection volum...

Embodiment 2

[0049]In order to verify the feasibility and accuracy of the detection method of Example 1, a precision test was carried out. The test adopted a concentration of 1000 mg / L of cyhalofop-ester and saflufenacil standard solution prepared, and 5 μL of the standard solution was continuously injected 6 times. The measurement results are shown in Table 2.

[0050] Table 2

[0051] serial number Cyhalofop-ethyl peak area The peak area of ​​saflufenacil 1 35305737 19577492 2 35378562 19629415 3 35309456 19584366 4 35287942 19507963 5 35432953 19459937 6 35407689 19566193 average value 35353723 19554228 RSD% 0.16 0.28

[0052] It can be drawn from Table 2 that the RSD of the peak area is less than 2%, so this method has good stability.

Embodiment 3

[0054] In order to better illustrate the stability of the sample solution containing cyhalofop-ethyl and saflufenacil provided in the examples of the present invention, the sample solution was prepared and placed for 4h, 8h, 12h, and 24h respectively, and then analyzed by liquid chromatography. The results are shown in Table 3.

[0055] table 3

[0056]

[0057]

[0058] As can be seen from Table 3, the RSD of the peak area is less than 2%, and the stability of the test solution provided by the present invention is good.

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Abstract

The invention provides a method for detecting the content of cyhalofop-methyl and saflufenacil in the compound preparation, which comprises: detecting the test sample of the compound preparation of cyhalofop-fop and saflufenacil through high performance liquid chromatography , to obtain the content of cyhalofop-methyl and the content of saflufenacil; wherein, the wavelength detected by the high performance liquid chromatography is selected to be 225-240nm in the present invention; the mobile phase detected by the high performance liquid chromatography is acetonitrile and water and the volume ratio of the acetonitrile and water is (58~62):(38~42), so that the method provided by the invention does not need to pre-preparate the compound preparation of cyhalofop-methyl and saflufenacil. The detection can be carried out after processing, and the detection result is accurate, the reproducibility is good, and the sensitivity is high.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to a method for detecting the content of cyhalofop-methyl and saflufenacil in compound preparations. Background technique [0002] Cyhalofop-ethyl and saflufenacil compound EC or compound oil suspension is a compound herbicide with remarkable herbicidal effect on gramineous weeds. At present, no relevant patents have been found for the method of determining the content of cyhalofop-ethyl and saflufenacil together at home and abroad, while the detection method for the composition of cyhalofop-fop is a forward detection method of high performance liquid chromatography, and the detection method for the composition of saflufenacil is a high-efficiency The liquid chromatography reversed-phase detection method, and the currently disclosed method takes a long time to detect each component separately and wastes reagents, and the impurity peak cannot be separated from the active component...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 孙国庆侯永生吴秀改王建娜张翠
Owner SHANDONG WEIFANG RAINBOW CHEM
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