Preparation method of refined egg yolk antibody for duck reovirus
A technology of reovirus and egg yolk antibody, which is applied in the field of preparation of duck reovirus refined egg yolk antibody, can solve problems such as ineffective treatment methods, achieve good prevention effect, reduce economic losses, and achieve significant economic and social benefits Effect
Inactive Publication Date: 2018-11-13
重庆永健生物技术有限责任公司
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[0004] The invention provides a preparation method of refined egg yolk antibody against duck reovirus, aiming to solve the problem that conventional treatment methods are basically ineffective
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[0023] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.
[0024] see figure 1 , the present invention provides a technical solution: a method for preparing duck reovirus refined yolk antibody, comprising the following steps:
[0025] Step S1, producing and selecting poisonous seeds according to the standard of poisonous seeds;
[0026] Step S2, inoculate the virus seed after dilution, and extract the antigen after testing;
[0027] Step S3, after the antigen is tested, it is converted into an immune antigen after concentration, oil phase preparation and water phase preparation, and after the immune antigen is tested and qualified, the immune antigen is ...
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The invention is applicable to the technical field of biology, and provides a preparation method of a refined egg yolk antibody for duck reovirus. The method comprises the following steps: S1, producing and selecting a virus variety according to the virus variety standard; S2, diluting the virus variety and then inoculating, testing and then extracting the antigen; according to the instructions for use, the refined egg yolk antibody has a prevention and protection rate of 99.79% when being used for'spleen and spleen necrosis' of ducks; when the refined egg yolk antibody is used for early, middle and late treatment of the'spleen and spleen necrosis' of the ducks, the survival rates are respectively increased by 7.28%, 5.82% and 2.21% compared with conventional treatment method. The refinedegg yolk antibody, prepared by the method, for the duck reovirus provides an effective means for the clinical prevention and treatment of the 'spleen and spleen necrosis', caused by the duck reovirus,of the ducks; compared with the conventional treatment method, the refined egg yolk antibody for the duck reovirus can remarkably reduce the economic loss brought to the duck breeding industry, thushaving significant economic benefit and social benefit.
Description
technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for preparing duck reovirus refined yolk antibody. Background technique [0002] Since March 2017, a kind of duckling has been popular in the farms of large-scale meat duck breeding groups in China. The clinical symptoms are soft feet, loose stools, and the pathological changes are yellowish necrosis in the liver and occasional bleeding points, and gray-white necrosis in the spleen. Or spleen enlargement and purple-black disease, which is clinically called duck "liver and spleen necrosis", and the causative pathogen is duck reovirus. All kinds of ducks, such as shelduck, white-feathered meat duck, hybrid duck, etc., are prone to occurrence, but cherry valley duck is more prone to occurrence. The age of onset is mostly within 9 days, the incidence rate is less than 30%, and the mortality rate within 7 days after onset is 10%. about [0003] Currently, there is no clinical a...
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IPC IPC(8): C07K16/10C07K16/06A61K39/42A61P31/14
CPCA61K2039/505A61P31/14C07K16/02C07K16/10C07K2317/11
Inventor 张立武赵光伟周宇邵骜俊李来旭张思旺李阁
Owner 重庆永健生物技术有限责任公司