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A biological veterinary drug for preventing and treating white diarrhea of piglets and a preparation method thereof

A technology for the prevention and treatment of piglet pullorum and biology, which is applied in the directions of biochemical equipment and methods, microorganism-based methods, microorganisms, etc., to achieve the effects of reducing drug resistance, reducing the frequency of use, and improving the function of the gastrointestinal tract

Active Publication Date: 2018-11-16
HUBEI WUDANG ANIMAL PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Piglet pullorum disease is usually based on prevention, and prevention is more important than cure. At present, there is no good preventive and therapeutic drug

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A biological veterinary drug for preventing and treating pullorum of piglets, which consists of the following components in terms of weight percentage: the concentration of bacteria is 10 9 Cfu / mL lactic acid bacteria fermentation broth 7%, bacterial concentration 10 9 Cfu / mL Clostridium butyricum fermentation broth 18%, bacterial concentration of 10 9 Cfu / mL Bacillus cereus fermentation broth 15%, the balance is water. The specific method is as follows:

[0030] (1) Preparation of liquid medium and expansion medium

[0031] In parts by weight, add 10 parts of starch, 10 parts of beef extract, 10 parts of peptone and 4 parts of NaCl into 1000 parts of water. After fully dissolving, add NaOH to adjust the pH to 7, filter, sterilize with damp heat and cool to 20°C. .

[0032] (2) Preparation of expansion medium containing poplar flower powder

[0033] Add 10 wt% poplar flower powder with a particle size of 80 mesh to the expansion medium prepared in step (1), and mix...

Embodiment 2

[0042] A biological veterinary drug for preventing and treating pullorum of piglets, which consists of the following components in terms of weight percentage: the concentration of bacteria is 10 8 Cfu / mL of lactic acid bacteria fermentation broth 10%, the bacterial concentration is 10 8 Cfu / mL Clostridium butyricum fermentation broth 25%, bacterial concentration 10 8 Cfu / mL Bacillus cereus fermentation broth 25%, the balance is water. The specific method is as follows:

[0043] (1) Preparation of liquid medium and expansion medium

[0044] In parts by weight, add 20 parts of starch, 20 parts of beef extract, 20 parts of peptone and 5 parts of NaCl into 2000 parts of water. After fully dissolving, add NaOH to adjust the pH to 7.5, filter, sterilize with damp heat and cool to 30°C. .

[0045] (2) Preparation of expansion medium containing poplar flower powder

[0046] Add 5 wt% poplar flower powder with a particle size of 100 mesh to the expansion medium prepared in step (1...

Embodiment 3

[0055] A biological veterinary drug for preventing and treating pullorum of piglets, which consists of the following components in terms of weight percentage: the concentration of bacteria is 10 7 Cfu / mL of lactic acid bacteria fermentation broth 10%, the bacterial concentration is 10 7 Cfu / mL Clostridium butyricum fermentation broth 15%, bacterial concentration 10 7 Cfu / mL Bacillus cereus fermentation broth 15%, the balance is water. The specific method is as follows:

[0056] (1) Preparation of liquid medium and expansion medium

[0057] In parts by weight, add 30 parts of starch, 30 parts of beef extract, 30 parts of peptone and 8 parts of NaCl into 3000 parts of water, add NaOH to adjust the pH to 7.5 after fully dissolving, filter, sterilize with damp heat and cool to 35°C. .

[0058] (2) Preparation of expansion medium containing poplar flower powder

[0059] Add 8 wt% poplar flower powder with a particle size of 120 mesh to the expansion medium prepared in step (1)...

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PUM

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Abstract

The invention relates to a biological veterinary drug for preventing and treating white diarrhea of piglets and a preparation method thereof, and belongs to the technical field of biological veterinary drugs. The biological veterinary drug includes, by weight, 5-10% of a lactic acid bacterium fermentation solution, 10-25% of a Clostridium butyricum fermentation solution and 10-25% of a Bacillus cereus fermentation solution, with the balance being water. The microbe concentrations of the three fermentation solutions are 10<7>-10<9> Cfu / mL. The Bacillus cereus fermentation solution is prepared by adding poplar flower powder into an enlargement culture medium of Bacillus cereus for fermentation culture of the Bacillus cereus, and preparing the Bacillus cereus fermentation solution after the fermentation culture is finished. The drug is characterized by being good in effect, safe, environmentally friendly, and the like. Through adoption of the drug, the number of times of use of chemical antibiotics can be reduced, thus reducing drug resistance of piglets to the chemical antibiotics. The drug is simple in preparation process, low in equipment requirement, low in cost and suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of biological veterinary drugs, and in particular relates to a biological veterinary drug for preventing and treating pullorum of piglets and a preparation method thereof. Background technique [0002] Pork is the main indicator of the vegetable basket project to improve the living standards of the people, and it is also an important factor affecting price fluctuations. Among them, the key to the development of the pig industry lies in the piglets. There used to be a saying that "well-raised piglets yield half of the income". Two months after the piglet is born, it is called the piglet period, and pullorum (also belonging to E. coli) is most prone to occur at this time. Piglets suffering from "pullourosis" are thin and small, refuse to eat, have diarrhea at first, and then turn into pullorum, contagious, intestinal mucosa has catarrhal inflammation, mesenteric lymph nodes are mildly swollen, and a few sick ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/744A61P1/12A61P31/04C12N1/20C12R1/145C12R1/085C12R1/01A61K35/742
CPCA61K35/742A61K35/744A61P1/12A61P31/04C12N1/20A61K2300/00
Inventor 殷素会陈国明熊琛
Owner HUBEI WUDANG ANIMAL PHARMA
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