Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of method for cultivating tissue culture seedlings of improving disease resistance of dendrobium officinale

A technology of dendrobium candidum and its cultivation method, which is applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problems affecting the efficacy of dendrobium candidum, improve disease resistance, shorten the growth cycle, and avoid the invasion of diseases and insect pests Effect

Active Publication Date: 2021-11-30
贵州卡农云服务有限公司
View PDF14 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] But in the above-mentioned method, indispensable bactericidal step, also suppressed the development of the endophyte of Dendrobium candidum and beneficial microorganism simultaneously, and then influenced the medicinal effect of Dendrobium candidum

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of method for cultivating tissue culture seedlings of improving disease resistance of dendrobium officinale
  • A kind of method for cultivating tissue culture seedlings of improving disease resistance of dendrobium officinale

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A method for cultivating tissue cultured seedlings for improving the disease resistance of Dendrobium officinale, comprising the steps of:

[0034] A protocorm induction: soak the capsules of Dendrobium officinale in the induction solution at a constant temperature of 18°C ​​for 12 hours;

[0035] B. Endophytic bacteria activation: the soaked capsules are placed in a blender, adding 0.5% of the capsule mass fermentation agent and 0.07% capsule mass of copper gluconate, stirring at a speed of 240r / min for 12min;

[0036] C Proliferation culture: sow the activated capsules on the purple sweet potato base cultivation substrate, and cultivate them at a constant temperature for 35 days at a temperature of 25°C;

[0037] D rooting culture: the sprouts after the multiplication culture are placed on the ore-based culture substrate, and cultivated naturally;

[0038] In the above method, the induction solution includes: guava 15kg, bitter gourd 7kg, celery 6kg, dandelion 17kg, ...

Embodiment 2

[0048]A method for cultivating tissue cultured seedlings for improving the disease resistance of Dendrobium officinale, comprising the steps of:

[0049] A protocorm induction: soak the capsules of Dendrobium officinale in the induction solution at a constant temperature of 10°C for 10 hours;

[0050] B Endophytic Activation: Put the soaked capsules in a blender, add 0.1% of the capsule mass fermentation agent and 0.04% capsule mass of copper gluconate, and stir for 5 minutes at a speed of 180r / min;

[0051] C Proliferation culture: sow the activated capsules on the purple sweet potato base cultivation substrate, and cultivate them at a constant temperature for 30 days at a temperature of 20°C;

[0052] D rooting culture: the sprouts after the multiplication culture are placed on the ore-based culture substrate, and cultivated naturally;

[0053] In the above method, the induction solution includes: guava 10kg, bitter gourd 3kg, celery 4kg, dandelion 12kg, water 25kg;

[005...

Embodiment 3

[0063] A method for cultivating tissue cultured seedlings for improving the disease resistance of Dendrobium officinale, comprising the steps of:

[0064] A protocorm induction: soak the capsules of Dendrobium officinale in the induction solution at a constant temperature of 15°C for 11 hours;

[0065] B. Endophytic bacteria activation: the soaked capsules are placed in a mixer, adding 0.4% of the capsule mass fermentation agent and 0.05% capsule mass of copper gluconate, stirring at a speed of 210r / min for 9 minutes;

[0066] C Proliferation culture: sow the activated capsules on the purple sweet potato base cultivation substrate, and cultivate them at a constant temperature for 32 days at a temperature of 22°C;

[0067] D rooting culture: the sprouts after the multiplication culture are placed on the ore-based culture substrate, and cultivated naturally;

[0068] In the above method, the induction solution includes: guava 13kg, bitter gourd 5kg, celery 5kg, dandelion 15kg, ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to seedling cultivation technology of Dendrobium officinale, in particular to a method for cultivating tissue cultured seedlings for improving the disease resistance of Dendrobium officinale, comprising steps: A. Protocorm induction: soaking dendrobium officinale capsules in an induction solution at a temperature of 10-18°C Soak at a constant temperature for 10-12 hours; B: Activation of endophytic bacteria: Put the soaked capsules in a blender, add fermentation bacteria and copper gluconate, and stir for 5-12 minutes at a speed of 180-240r / min; C Proliferation culture: Sow the activated capsules on the purple sweet potato-based cultivation medium, and cultivate them at a constant temperature for 30-35 days at a temperature of 20-25°C; Adopting the seedling raising method of Dendrobium candidum of the present invention is easy to operate, green and pollution-free, has a high survival rate, improves the disease resistance of Dendrobium candidum, and the transplanting survival rate is as high as 99.5%.

Description

technical field [0001] The invention relates to a technique for growing seedlings of Dendrobium candidum, in particular to a method for cultivating tissue-cultured seedlings for improving the disease resistance of Dendrobium candidum. Background technique [0002] Dendrobium officinale is a perennial herb of the genus Dendrobium orchidaceae. It contains polysaccharides, mannitol, various alkaloids, various amino acids, volatile substances, trace elements and mucilage, etc. It can enhance body immunity, delay aging, relieve fatigue, and resist oxidation. , lowering blood pressure, lowering blood sugar, promoting digestion, promoting saliva secretion, anti-tumor, reducing phlegm and relieving cough, preventing radiation damage and many other functions. As wild Dendrobium candidum is becoming less and less and is on the verge of extinction, but the demand is increasing day by day, artificial planting is particularly important, but most of the sources of artificially cultivated ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 邱吉贵
Owner 贵州卡农云服务有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com