A polydopamine-wrapped gold core/hollow silicon shell nanomaterial and its preparation and application
A nanomaterial, polydopamine technology, applied in nanotechnology, metal processing equipment, preparations for in vivo experiments, etc., can solve the problems of difficult control of shape and uneven loading of nano gold stars, and achieve controllable size and shape , good practical value, and simple preparation method
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Embodiment 1
[0089] The specific process of preparing the ASPP nano-platform is as follows: figure 1 shown.
[0090] (1) Take 30ml of 1mM chloroauric acid solution and heat it to 100°C while stirring, then quickly inject 3ml of sodium citrate solution with a mass fraction of 1%, continue heating and reflux for 20-30min, and cool the solution to room temperature , to obtain a gold colloidal solution. into the gold colloidal solution to facilitate subsequent coating of the silica layer, and stirred and reacted at room temperature for 24 hours. The product was collected by centrifugation.
[0091] (2) Add 0.235ml polyvinylpyrrolidone solution (12.8g / L) to the gold colloid solution in (1), and stir at room temperature for 24h to obtain PVP-modified gold nanoparticles, which are collected by centrifugation.
[0092] (3) Disperse the product obtained in (2) into a mixture of ammonia water (0.62ml), absolute ethanol (13.6ml) and deionized water (3.3ml), ultrasonically disperse for 10min, then...
Embodiment 2
[0098] The product obtained in (7) in Example 1 was configured with sterile PBS buffer to form a mother solution with a gold concentration of 48 μg / ml, and then serially diluted to 32, 16, 8, 4, and 2 μg / ml. The cultured 4T1 cells were planted in a 96-well plate, and inoculated at a density of 10,000 cells / well, with a volume of 100 μL per well. After culturing overnight, wash 2-3 times with PBS, then add the above-mentioned materials of each dilution gradient, and co-culture with the cells for 24 hours. Five parallel wells were made for each gradient, and PBS buffer was used as a blank control. After the culture, wash with 100 μL PBS three times, then add 90 μL serum-free medium and 10 μL CCK8 solution to each well, incubate at 37 °C for 2 h, and detect the absorbance at 450 nm with a microplate reader. The results of cell viability detected by CCK-8 method showed that ASPP did not show obvious cytotoxicity and showed good cytocompatibility ( Figure 6 ).
Embodiment 3
[0100] The product obtained in (7) in Example 1 was prepared with ultrapure water into materials with a gold concentration of 20mM and 40mM, filled with 2mL PE tubes respectively, and B-mode ultrasonic imaging tests were performed on the two groups of materials. Ultrapure water was used as blank control. The test results show that: within the test concentration range, ASPP exhibits excellent US imaging effects ( Figure 7 ).
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