Unlock instant, AI-driven research and patent intelligence for your innovation.

Organic-inorganic composite immobilized cell and its preparation method and application

A technology of immobilized cells and inorganic composites, applied in biochemical equipment and methods, immobilized enzymes, immobilized on or in inorganic carriers, etc., to achieve the effects of low cost, enhanced tolerance, and excellent stability

Active Publication Date: 2021-03-30
JIANGNAN UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] But so far, adding inorganic materials to organic immobilized cells and applying them to nitrilase biotransformation has not been reported in any literature

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Organic-inorganic composite immobilized cell and its preparation method and application
  • Organic-inorganic composite immobilized cell and its preparation method and application
  • Organic-inorganic composite immobilized cell and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The preparation of embodiment 1 bacterial suspension

[0037] The nitrilase-producing mutant mut-D3 was inoculated in 50 mL of liquid medium, and cultured with shaking at 30° C. for 20-36 h, preferably 24 h in this embodiment, as the seed liquid. Transfer to fresh fermentation medium with 1-5% inoculum size and shake culture at 30°C for 30-45h. In this embodiment, the preferred inoculum size is 1%, and the culture time is 40 h. Centrifuge at 8000 rpm for 10 min at 4°C to remove the supernatant and collect the cells. Use 100mM, pH7.2 sodium phosphate buffer to wash 2-3 times, preferably 2 times in this embodiment, and resuspend the bacterium in the buffer to make a 20mg / mL bacterial suspension, place in 4 ℃ refrigerator for later use.

Embodiment 2

[0038] Example 2 Preparation of organic-inorganic composite immobilized cells

[0039] This implementation provides a method for preparing organic-inorganic composite immobilized cells, adding inorganic materials SiO2 to sodium alginate-PVA immobilized cells 2 , CaCO 3 , bentonite, to investigate the influence of a single inorganic material on the performance of immobilized cells, the specific method is as follows:

[0040] The sodium alginate aqueous solution with a mass concentration of 2.5% and the PVA aqueous solution with a mass concentration of 8% were mixed according to a ratio (volume ratio) of 9:1 as an organic fixing material. The above-mentioned organic fixing material was mixed with the bacterial suspension prepared in Example 1 (volume ratio) at 1:1 to obtain a composite organic material solution. Then add different inorganic materials to the composite organic material solution: SiO 2 (1.0%, 1.5%, 2.0%, 2.5%, 3.0%), CaCO 3 (0.2%, 0.4%, 0.6%, 0.8%, 1.0%), bento...

Embodiment 3

[0043] Example 3 Preparation of organic-inorganic composite immobilized cells

[0044] This implementation provides a preparation method of organic-inorganic composite immobilized cells, adding different concentrations of inorganic material SiO to sodium alginate-PVA immobilized cells at the same time 2 , CaCO 3 , to investigate the effect of composite inorganic materials on immobilized cells, the specific method is as follows:

[0045] The sodium alginate aqueous solution with a mass concentration of 2.5% and the PVA aqueous solution with a mass concentration of 8% were mixed according to a ratio (volume ratio) of 9:1 as an organic fixing material. The above-mentioned organic fixing material was mixed with the bacterial suspension prepared in Example 1 (volume ratio) at 1:1 to obtain a composite organic material solution. Add 1.5% SiO to the composite organic material solution 2 +0.6%CaCO 3 , 1.5% SiO 2 +0.8%CaCO 3 , 1.5% SiO 2 +1.0%CaCO 3 , 2.0% SiO 2 +0.6%CaCO 3 ,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a preparation method of an organic-inorganic composite immobilized cell. The preparation method comprises steps as follows: after a bacterial suspension of nitrilase producingbacteria and an organic material water solution are mixed uniformly, the mixture is mixed with an inorganic material, cell immobilization is performed in an immobilization solution at the temperatureof 2-5 DEG C, and the organic-inorganic composite immobilized cell is obtained, wherein the concentration of the bacterial suspension is 10-40 mg / mL; organic materials are sodium alginate and polyvinyl alcohol. The invention also provides a biosynthesis method of niacin. The prepared organic-inorganic composite immobilized cell and a substrate 3-cyanopyridine are mixed, the pH of the mixed solution is subjected to a reaction under the conditions of the pH of 7.5-8.5 and the temperature of 20-55 DEG C for 30-60 min, and niacin is prepared through reaction in multiple batches. The provided immobilization method is simple to operate and low in cost, and the immobilized cell has high mechanical strength, good mass transfer performance, high heat stability and high recycling rate and providesa helpful thought for industrial application.

Description

technical field [0001] The invention relates to the field of industrial biotechnology, in particular to an organic-inorganic composite immobilized cell and its preparation method and application. Background technique [0002] Nitrilase contains a catalytic triad (Glu-Lys-Cys), which can catalyze nitrile compounds to the corresponding carboxylic acid and ammonia in one step. However, most of the nitrile compounds have certain cytotoxicity and can inhibit the activity of nitrilase, thereby causing cell degeneration and lysis. Free cell catalysts will directly suffer from the toxicity of nitrile substrates during the conversion process, while cell immobilization technology can not only improve the reaction stability, thermal stability, storage stability, and operation stability of cell catalysts, but also to a certain extent On the one hand, it can reduce the toxicity of the cells to the substrate, and it is beneficial to the continuous treatment of the reaction of carboxylic ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14C12N11/10C12N11/084C12P17/12
CPCC12N9/78C12N11/08C12N11/10C12N11/14C12P17/12C12Y305/05001
Inventor 许正宏史劲松龚劲松董婷婷李恒
Owner JIANGNAN UNIV