Epinephelus coioides antimicrobial peptide and application thereof

A technology of oblique grouper and antibacterial peptides is applied in the field of genetic engineering research and development to achieve the effects of reducing production costs, enriching gene pools and having broad application prospects.

Active Publication Date: 2018-11-27
SOUTH CHINA NORMAL UNIVERSITY
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  • Abstract
  • Description
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Problems solved by technology

Although some scholars have studied the CD59 protein in fishes such as zebrafish (Sun et al., 2013), Nile tilapia (Gan et al. 2015), large yellow croaker, rainbow trout and channel catfish (Liu et al, 2007; Papanastasiou et al. al,2007; Yeh et al,2007), but there is no report on the use of the grouper CD59 protein to inhibit the growth of Vibrio alginolyticus

Method used

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  • Epinephelus coioides antimicrobial peptide and application thereof
  • Epinephelus coioides antimicrobial peptide and application thereof
  • Epinephelus coioides antimicrobial peptide and application thereof

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Embodiment Construction

[0034] The present invention will be further described below in conjunction with specific experiments. It should be understood that the following experiments are only used to illustrate the present invention and are not intended to limit the protection scope of the present invention.

[0035] The molecular biology experimental techniques used in the following examples include PCR amplification, plasmid extraction, plasmid transformation, DNA fragment ligation, enzyme digestion, gel electrophoresis, etc., unless otherwise specified, generally operate according to conventional methods. For details, please refer to " Molecular Cloning Experiment Guide (Third Edition) (translated by Sambrook J, Russell DW, Janssen K, Argentine J. Huang Peitang, etc., 2002, Beijing: Science Press), or according to the conditions suggested by the manufacturer.

[0036] 1. Obtaining the ORF of the CD59 gene of the oblique-banded grouper by designing merger primers

[0037]According to the conservatio...

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Abstract

The invention discloses an epinephelus coioides antimicrobial peptide. The antimicrobial peptide is epinephelus coioides CD59 protein, and has an amino acid sequence as shown in SEQ ID NO.1, or protein having an amino acid sequence as shown in SEQ ID NO.1, which is substituted, lost and / or added with one or more amino acids and / or is terminal modified and has the same or higher activity. Experiments prove that the epinephelus coioides antimicrobial peptide can be used for inhibiting the growth activity of vibrio alginolyticus, can be applied to preparation of immune stimulant or feed additivesfor fish, especially groupers, and has a wide application prospect. The CD59 gene sequence of the epinephelus coioides is obtained for the first time, can be used for enriching the gene pool of groupers, can be applied to preparation of recombinant protein, antibacterial and bacteriostatic additive and fish immune simulants, and provides new practice foundation for physiological immune researchesof epinephelus coioides.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering research and development, and in particular relates to an antimicrobial peptide of grouper and its application. Background technique [0002] Slanted grouper (Epinepheluscoioides) belongs to Perciformes (Perciformes), Serranidae (Serranidae), grouper subfamily (Epinephelinae), grouper (Epinephelus), is a warm water reef fish, in the Indian Ocean Widely distributed in tropical and subtropical waters of the Pacific Ocean. Grouper is one of the famous and precious marine fishes. Its meat is delicious, and it is loved by consumers all over the world, and its economic value is huge. However, due to the current rapid climate change disasters and seriously polluted water quality problems, the morbidity and mortality rates remain high, which has caused serious economic blows to the artificial breeding of grouper. Vibrio alginolyticus is a common pathogenic bacteria. After the grouper is infe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/46C12N15/12A61K38/17A61P37/06A23K20/147A23K50/80
CPCA61K38/00A23K20/147A23K50/80A61P37/06C07K14/461
Inventor 王维娜罗盛伟魏巍杨萍刘媛
Owner SOUTH CHINA NORMAL UNIVERSITY
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