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Hybridization breeding method of Elopichthys bambusa and Megalobrama amblycephala

A technology for cross-breeding and bream, applied in fish farming, application, climate change adaptation, etc., can solve the problems of asynchrony and mutual inhibition of time and space order, disordered expression, and the inability of offspring to survive normally.

Active Publication Date: 2018-12-04
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] (3) Sexual or behavioral isolation Due to the difference in mating behavior, it is difficult for two or several groups with close kinship to mate successfully
In some hybrid combinations, although the number of chromosomes of the parents is equal, but the karyotypes of the chromosomes are different and other reasons, the expression of the alleles after the sperm-egg pairing is disordered, resulting in the offspring not being able to survive normally.
[0010] 3. Differences in the gene locus or expression sequence of time and space of the enzyme. The farther the parental relationship of the hybrid is, the time and space sequence of the expression of the alleles of the parents may be out of sync or mutually inhibited [8]
The bream and bream belong to different subfamilies, and there are big differences between the two in terms of genetics, taxonomy, and body shape. So far, there has been no successful distant hybridization between bream and bream at home and abroad. Therefore, it is of great application value to explore a method of hybridization between bream and bream and to study and analyze the biological characteristics of their offspring

Method used

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  • Hybridization breeding method of Elopichthys bambusa and Megalobrama amblycephala
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  • Hybridization breeding method of Elopichthys bambusa and Megalobrama amblycephala

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Embodiment 1

[0057] A method for hybrid breeding of bream and bream, comprising the steps of:

[0058] (1) The parents of bream are healthy and sexually mature male bream from the aquaculture base of Dongfeng Farm, Daye City, Hubei Province, and the broodstock are healthy and sexually mature bream from the original seed farm of Ezhou City, Hubei Province. The 3-4 year old female bream with a body weight of more than 1.0Kg in the early stage is respectively carried out to strengthen the cultivation of the parents.

[0059] (2) Artificial oxytocin of the female parents of bream: In mid-May, two injections were used to inject oxytocin drugs into the parents of bream, and the first injection of gonadotropin-releasing hormone analogs (GnRH-A 2 )1μg / Kg, the second injection is GnRH-A 2 4μg / Kg + dioconone 4mg / Kg, needle distance 8-10h. Put the bream that has been injected with the oxytocic agent into the oxytocic pool.

[0060] (3) Artificial insemination: Squeeze bream eggs into a basin fille...

Embodiment 2

[0065] A method for hybrid breeding of bream and bream, except for step (3), all the other steps are exactly the same as those in Example 1. In this embodiment, the sperm of bream that was frozen and revived was used in step (3).

[0066] The fertilization rate of recovered sperm after hybridization was 25%, the hatching rate was 15%, and the survival rate was 35%.

[0067] The spermatozoa in this embodiment were cryopreserved and revived in the following manner:

[0068] (1) Semen collection: collect fresh semen from broodstock with good maturity and store it in a clean environment at 4°C;

[0069] The fresh semen of the broodstock, the normal semen is milky white when observed by the naked eye, is activated with clear water and is used for preservation of the semen with an average survival rate greater than 75%;

[0070] (2) Diluent preparation: preparation containing 7.5g / LNacl, 0.3g / LKCl, 0.4g / LNaHCO 3 , 3g / L glucose solution, adjust the pH to 6.5, and place it in a 4°C ...

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Abstract

The invention belongs to the technical field of hybridization breeding methods of fishes, and relates to a hybridization breeding method of Elopichthys bambusa and Megalobrama amblycephala. Hybridization breeding is conducted on eggs of female Megalobrama amblycephala and sperms of male Elopichthys bambusa, and the obtained hybridized F1 has fast growth, delicate flesh, omnivorous feeding habits,strong resistance and strong disease resistance. The hybridization breeding method has strong operability and good adaptability; and in the hybridization process, the fertilization rate can reach 55%,the hatching rate can reach 40%, and the survival rate of fry can reach 65%; most of characteristics of the hybridized fishes are partial to those of Megalobrama amblycephala, and compared with Megalobrama amblycephala, the hybridized fishes have certain growth advantages and feeding advantages, and are suitable for large-scale breeding.

Description

technical field [0001] The invention belongs to the technical field of fish hybrid breeding, and in particular relates to a method for hybrid breeding of bream and bream. Background technique [0002] Distant hybridization refers to the hybridization between different species, which can be interspecific, intergeneric or even more distantly related species. Distant hybridization can combine the biological characteristics of different individuals belonging to different species, genera and other distant genetic relationships, break through the boundaries between species, expand genetic variation, and create new varieties and even new populations with genetic variation, fertility, and excellent traits. important method of [1] . In terms of phenotype, distant hybridization can integrate the advantages of both parents, making the offspring show heterosis in terms of appearance, growth speed, survival rate and disease resistance. In terms of genotype, distant hybridization leads...

Claims

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Application Information

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IPC IPC(8): A01K61/10
CPCA01K61/10Y02A40/81
Inventor 王卫民陈静郭竞王赛
Owner HUAZHONG AGRI UNIV
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