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Applications of PF4 in resisting RSV

A technology of application and platelet factor, which can be used in medical preparations containing active ingredients, antiviral agents, peptide/protein components, etc., which can solve the problem that the antiviral function of PF4 is rarely studied.

Active Publication Date: 2018-12-04
INST OF PATHOGEN BIOLOGY CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Little research has been done on the antiviral function of PF4

Method used

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  • Applications of PF4 in resisting RSV
  • Applications of PF4 in resisting RSV
  • Applications of PF4 in resisting RSV

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Example 1.PF4 inhibits the infection and replication of RSV in HEp-2 cells

[0076] 1.1 Experimental scheme

[0077] Inoculate HEp-2 cells (purchased from ATCC, number: ATCC CCL-23, cell concentration 1.8×10 5 cells / ml) in 96-well and 24-well cell plates, placed at 37°C in 5% CO 2 Cultivate for 16 h, use recombinant human PF4 protein (ExCell Bio, product number: CB055-492 ) to treat HEp-2 cells at 37°C with 5% CO 2 After incubation for 2 h, continue to incubate with PF4 mixture with MOI (multiplicity of infection) = 1 (96-well plate) or 2 (24-well plate) and PF4 mixture, mix The final concentration of PF4 in the solution was the same as before; replace it with maintenance solution (Opti-MEM medium containing 2% fetal bovine serum and 1% penicillin-streptomycin, wherein fetal bovine serum was purchased from Hyclone Company, article number: SH30396.03; green chain Mymycin was purchased from Gibco, Cat. No.: 15140-122; Opti-MEM medium was purchased from Gibco, Cat. No....

Embodiment 2

[0082] Example 2.PF4 reduces lung viral load and lung inflammation level after RSV infection in mice

[0083] 2.1 Experimental scheme

[0084] SPF grade 6-8 week-old female BALB / c mice (Beijing Huafukang Biotechnology Co., Ltd.) were reared in a BSL-2 environment after passing the quarantine, with 4 to 6 mice per cage, and sufficient food and drinking water supply; the experiment began The former mice need to be fed for more than 3 days to fully adapt to the environment. The mice were randomly divided into 3 groups, 8 mice in the RSV+PF4 group, 8 mice in the RSV group, and 5 mice in the control group. The mice in the RSV+PF4 group and the RSV group were intraperitoneally injected with 1% sodium pentobarbital 125 μl / only. After the mice were anesthetized, each mouse in the RSV+PF4 group was given 1.8 μg of recombinant human PF4 protein dissolved in DPBS by intranasal drops ( About 0.1mg PF4 / kg body weight), solution volume 18μl; each mouse in RSV group was given 18μl DPBS int...

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Abstract

The invention discloses applications of PF4 in resisting RSV. According to the applications, platelet factor 4 can alleviate the virus infection symptom by inhibiting the respiratory syncytial virus replication in the body of a host, and the technical scheme is an effective intervening measure for resisting respiratory syncytial virus infection. The invention provides applications of the plateletfactor 4 in preparing medicines for preventing and / or treating diseases and / or symptoms caused by respiratory syncytial virus infection, and further provides administration paths and dosage forms suitable for the medicines, and medicine combination of the medicines with outer medicines. In addition, the invention further provides a method with non-treatment purpose for inhibiting the respiratory syncytial viruses in in-vitro cells by adopting the platelet factor 4.

Description

technical field [0001] The present invention relates to respiratory syncytial virus, and in particular to antiviral interventions useful for the prevention and / or treatment of diseases and / or symptoms caused by the virus. Background technique [0002] Respiratory syncytial virus (Respiratory Syncytial Virus, RSV) belongs to Paramyxoviridae Pneumoviridae subfamily Pneumovirus, is an enveloped single-stranded negative-sense RNA virus, the genome encodes a total of 11 viral proteins, from 3' to 5' Nonstructural protein 1 (NS1), nonstructural protein 2 (NS2), nucleocapsid protein (N), phosphoprotein (P), matrix protein (M), small hydrophobin (SH), glycoprotein (G) , fusion protein (F), M2ORF1 / ORF2 protein (M2-1 / M2-2) and large protein (L). RSV is distributed worldwide and has only one serotype. It can be divided into two subgroups, A and B, according to antigen reactivity. Subgroup A is prevalent in most parts of the world (Griffiths C, Drews S J, Marchant D J. Respiratory Sync...

Claims

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Application Information

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IPC IPC(8): A61K38/19A61K45/06A61P31/14
CPCA61K38/195A61K45/06A61K2300/00
Inventor 王健伟任丽丽韩子泊
Owner INST OF PATHOGEN BIOLOGY CHINESE ACADEMY OF MEDICAL SCI
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