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Anti-RSV application of pf4

A technology of application and platelet factor, which can be used in medical preparations containing active ingredients, antiviral agents, peptide/protein components, etc., which can solve the problem that the antiviral function of PF4 is rarely studied.

Active Publication Date: 2021-12-03
INST OF PATHOGEN BIOLOGY CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Little research has been done on the antiviral function of PF4

Method used

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  • Anti-RSV application of pf4
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  • Anti-RSV application of pf4

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Example 1.PF4 inhibits the infection and replication of RSV in HEp-2 cells

[0076] 1.1 Experimental scheme

[0077] Inoculate HEp-2 cells (purchased from ATCC, number: ATCC CCL-23, cell concentration 1.8×10 5 cells / ml) in 96-well and 24-well cell plates, placed at 37°C in 5% CO 2 Cultivate for 16 h, use recombinant human PF4 protein (ExCell Bio, product number: CB055-492 ) to treat HEp-2 cells at 37°C with 5% CO 2 After incubation for 2 h, continue to incubate with PF4 mixture with MOI (multiplicity of infection) = 1 (96-well plate) or 2 (24-well plate) and PF4 mixture, mix The final concentration of PF4 in the solution was the same as before; replace it with maintenance solution (Opti-MEM medium containing 2% fetal bovine serum and 1% penicillin-streptomycin, wherein fetal bovine serum was purchased from Hyclone Company, article number: SH30396.03; green chain Mymycin was purchased from Gibco, Cat. No.: 15140-122; Opti-MEM medium was purchased from Gibco, Cat. No....

Embodiment 2

[0082] Example 2.PF4 reduces lung viral load and lung inflammation level after RSV infection in mice

[0083] 2.1 Experimental scheme

[0084] SPF grade 6-8 week-old female BALB / c mice (Beijing Huafukang Biotechnology Co., Ltd.) were reared in a BSL-2 environment after passing the quarantine, with 4 to 6 mice per cage, and sufficient food and drinking water supply; the experiment began The former mice need to be fed for more than 3 days to fully adapt to the environment. The mice were randomly divided into 3 groups, 8 mice in the RSV+PF4 group, 8 mice in the RSV group, and 5 mice in the control group. The mice in the RSV+PF4 group and the RSV group were intraperitoneally injected with 1% sodium pentobarbital 125 μl / only. After the mice were anesthetized, each mouse in the RSV+PF4 group was given 1.8 μg of recombinant human PF4 protein dissolved in DPBS by intranasal drops ( About 0.1mg PF4 / kg body weight), solution volume 18μl; each mouse in RSV group was given 18μl DPBS int...

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Abstract

The anti-RSV application of PF4 discloses that the platelet factor 4 can alleviate the symptoms of virus infection by inhibiting the replication of respiratory syncytial virus in the host body, and is an effective anti-respiratory syncytial virus infection intervention measure. The invention provides the use of platelet factor 4 in the preparation of medicines for preventing and / or treating diseases and / or symptoms caused by respiratory syncytial virus infection, and also provides administration routes, dosage forms and medicines suitable for the medicines. Combination with other drugs, etc.; In addition, it also provides a non-therapeutic method for inhibiting respiratory syncytial virus in cells in vitro with platelet factor 4.

Description

technical field [0001] The present invention relates to respiratory syncytial virus, and in particular to antiviral interventions useful for the prevention and / or treatment of diseases and / or symptoms caused by the virus. Background technique [0002] Respiratory syncytial virus (Respiratory Syncytial Virus, RSV) belongs to Paramyxoviridae Pneumoviridae subfamily Pneumovirus, is an enveloped single-stranded negative-sense RNA virus, the genome encodes a total of 11 viral proteins, from 3' to 5' Nonstructural protein 1 (NS1), nonstructural protein 2 (NS2), nucleocapsid protein (N), phosphoprotein (P), matrix protein (M), small hydrophobin (SH), glycoprotein (G) , fusion protein (F), M2ORF1 / ORF2 protein (M2-1 / M2-2) and large protein (L). RSV is distributed worldwide and has only one serotype. It can be divided into two subgroups, A and B, according to antigen reactivity. Subgroup A is prevalent in most parts of the world (Griffiths C, Drews S J, Marchant D J. Respiratory Sync...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/19A61K45/06A61P31/14
CPCA61K38/195A61K45/06A61K2300/00
Inventor 王健伟任丽丽韩子泊
Owner INST OF PATHOGEN BIOLOGY CHINESE ACADEMY OF MEDICAL SCI
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