Method for utilizing brachymeria nosatoi Habu for preventing and controlling pisina arenosella Walker
A technology of coconut weaving moth and diamond drill, applied in the fields of botanical equipment and methods, application, monitoring of invasive species, etc., can solve problems such as environmental pollution, killing natural enemies, and rampant pests.
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Embodiment 1
[0022] Embodiment 1: the expansion and multiplication of host and D. diamonda
[0023] (1) Expansion of the host: collect a certain amount of coconut moths of various worm states from the outdoor coconut tree, prepare the breeding chamber, the breeding cage, and feed the coconut leaves to multiply;
[0024] (2) Propagation of the diamond-shaped wasp: select the diamond-shaped wasp that has just emerged for 2 days (the male-to-female ratio of the bee is 1:1); use the pupa of the coconut moth (not more than 5 days old) as the host to multiply the diamond Dip the nutrient source with a cotton ball and put it in the finger tube. Let the male and female bees mate for 24-48 hours, then add the host (the ratio of seed bee to host is 2:5) to parasitize for 24 hours, then take out the female bee and continue Carry out the parasitism of other pupae until the female bee dies, supplement nutrition once a day, and generate one generation in 17-22 days. The culture conditions were 30°C, re...
Embodiment 2
[0026] Embodiment 2: the selectivity test of D. diamondia to different day-old cocoa moth pupae
[0027] Introduce 20 adult bees that have been eclosion for 1 day (the ratio of male and female bees is 1:1) into the bee breeding cage, let the male and female bees mate for 24 days, and then put 50 adults for 1, 2, 3, 4, 5, 6, 8, and 10 days The petri dishes of the pupae of the young coconut moth were placed radially in the breeding cages, supplemented with cotton balls stained with nutrient sources, parasitized for 24 hours, and each treatment was repeated 3 times. The parasitism rate of the wasps diamondatum on pupae of different day-old cocoon moths were counted.
[0028] Table 1 The parasitism rate of D. diamantanus on pupae of different day-old cocoon moths
[0029] Pupal age of coconut weaver moth
[0030] It can be seen from Table 1 that the effect of parasitizing the 1 to 4-day-old cocoon moth pupae of the wasp is the best, and the parasitic rate decreases afte...
Embodiment 3
[0031] Embodiment 3: the influence of different culture conditions on parasitic rate
[0032] Introduce 20 adults (female to male ratio 1:1) that have been eclosion for 1 day into the breeding bee cage, and the culture conditions are shown in Table 2. After the male and female bees are mated for 24 hours, 50 2-day-old coconut moth pupae are placed in the petri dish Place them in the beehive cages, supplement nutrition with cotton balls stained with nutrient sources, parasitize for 24 hours, and repeat 3 times for each treatment. The parasitism rate of the wasps diamondatum on pupae of different day-old cocoon moths were counted.
[0033] Table 2 Effect of different culture conditions on parasitism rate
[0034]
[0035] It can be seen from Table 2 that the parasitizing conditions of the wasp D. adamantine are that the temperature is 20-30° C., and when the humidity is 70-80%, the parasitism rate reaches more than 90%.
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