Solid culture medium for production of cordyceps powder and production method of cordyceps powder

A technology of solid medium and Cordyceps fungus powder, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of unstable product quality, low raw material utilization rate, single technical solution, etc., and achieve easy scale-up The effect of large-scale industrial production

Pending Publication Date: 2018-12-11
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The Cordyceps fermentation method disclosed in the prior art has the defects of single technical scheme, low utilization rate of raw materials, and unstable product quality. In order to overcome the above defects, a new fermentation method of Cordyceps powder is urgently needed to meet the needs of large-scale production. requirements

Method used

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Examples

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Comparison scheme
Effect test

preparation example Construction

[0045] The present invention provides a preparation method of Cordyceps micropowder. In the present invention, the Cordyceps fungus powder obtained in the above scheme is dried to obtain dried fungus powder; the drying temperature is preferably 50-70° C., more preferably 60°C; the drying time is preferably 22 to 26 hours, more preferably 24 hours; the drying equipment is preferably an electric heating constant temperature blast drying oven.

[0046] In the present invention, the obtained dried bacteria powder is subjected to cell crushing to obtain Cordyceps fungus powder micropowder; the crushing equipment is preferably a Pallas cell crusher, and the speed of the Pallas cell crusher is preferably 48000r / min ; The power of the Pallas cell crusher is preferably 850w; the crushing time is preferably 2min. The cell crushing treatment can destroy the cell wall and fully release the effective substances. Therefore, compared with the Cordyceps fungus powder, the nutrients and active...

Embodiment 1

[0049] Embodiment 1 A kind of preparation method of the solid culture medium of producing Cordyceps fungus powder

[0050] Take 10g of glucose, 2.5g of peptone, 0.5g of yeast powder, 0.25g of potassium dihydrogen phosphate, 0.25g of magnesium sulfate, 486.5g of No. 3 corn grains and 500mL of pure drinking water, mix them well, and put them in a size of 18*33cm 2 Sterilize at 121°C for 20 minutes at 121°C for 20 minutes; after 24 hours, sterilize at 121°C for 20 minutes; after cooling at room temperature, perform aseptic operation to remove part or the whole of the solidified culture medium , put it upside down in a new sterile petri dish, and obtain a solid medium for producing Cordyceps fungus powder.

Embodiment 2

[0051] Embodiment 2 A kind of preparation method of the solid culture medium of producing Cordyceps fungus powder

[0052] 1) Take 10g of glucose, 2.5g of peptone, 0.5g of yeast powder, 0.25g of potassium dihydrogen phosphate, 0.25g of magnesium sulfate and 500mL of water, mix them, and heat them at 100°C for 1min to fully dissolve them to obtain a dissolved culture base;

[0053] 2) Take a 9cm glass petri dish, put 10g No. 3 corn granules and 1g agar in each dish, then add the dissolved medium obtained in step 1), and sterilize at 121°C for 20min; after 24h, re- Sterilize for 20 minutes; after cooling at room temperature, perform aseptic operation, take out part or the whole of the solidified medium, and put it upside down into a new sterile petri dish to obtain a solid plate medium for producing Cordyceps fungus powder.

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Abstract

The invention provides a solid culture medium for production of cordyceps powder and a production method of cordyceps powder, and belongs to the technical field of cordyceps powder. The production method of the cordyceps powder comprises the following steps of initially inoculating cordyceps into a liquid culture medium to perform basic culture, so as to obtain a basic culture liquid; transferringthe basic culture liquid into the other liquid culture medium to perform basic culture for the second time, so as to obtain a fermenting seed liquid; inoculating the fermenting seed liquid into a solid culture medium to perform amplification culture, and collecting the cordyceps powder, wherein the cordyceps is hirsutella sinensis. The production method of the cordyceps powder using corn flour asthe main raw material of the culture medium has the advantages that the large-scale industrialized production is easy, and the cordyceps powder can be quickly, efficiently, green and safely produced;the cordyceps powder produced by the method has good effect on treating the bronchitis and the cough caused by deficiency of the lung and kidney.

Description

technical field [0001] The invention relates to the technical field of production of Cordyceps powder, in particular to a solid medium for producing Cordyceps powder and a method for producing Cordyceps powder. Background technique [0002] Cordyceps sinensis is the Ergotaceae fungus Cordyceps sinensis fungus, also known as Cordyceps fungus, is a dry complex of sub-seats and larval corpses that parasitize on the larvae of bat moths. Cordyceps sinensis is mainly distributed in my country's Tibet, Qinghai, Sichuan, Yunnan and Gansu provinces, as well as India, Nepal, Bhutan and other areas above 3000 meters above sea level and below the snow line. Cordyceps sinensis contains a variety of active ingredients, and its medicinal value has attracted widespread attention. However, artificial cultivation of Cordyceps sinensis is extremely difficult, and wild resources are scarce. The production of natural Cordyceps sinensis is limited and expensive, the growth is slow, and the culti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A61K36/068A61P11/00A61P11/14C12R1/645
CPCA61K36/068A61P11/00A61P11/14C12N1/14
Inventor 沙涛张亚平
Owner YUNNAN UNIV
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