Dry chemical test paper for neuraminidase and preparation method thereof

A sialidase and dry chemical technology, applied in the field of sialidase dry chemical test paper and its preparation, can solve the problems of poor specificity of polyamine detection kits, and achieve the objective of diagnosing BV indicators, suitable for popularization and simple operation. Effect

Inactive Publication Date: 2018-12-11
DIRUI MEDICAL TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The purpose of the present invention is to solve the technical problem of the poor specificity of the polyamine detection kit used to detect bacterial vaginosis in the prior art, to provide a dry chemical test paper for sialidase detection and its preparation method

Method used

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  • Dry chemical test paper for neuraminidase and preparation method thereof
  • Dry chemical test paper for neuraminidase and preparation method thereof

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preparation example Construction

[0039] The present invention also provides a method for preparing sialidase detection dry chemical test paper, the steps are as follows:

[0040] Step 1, dissolving the substrate in a buffer solution containing metal ions, adding a surfactant to form an immersion solution A, soaking the filter paper with the immersion solution A, and drying to obtain a filter paper dispersed with the substrate;

[0041] Step 2, dissolving the chromogen in an alcohol solvent to form an immersion liquid B, soaking the filter paper dispersed with the substrate obtained in step 1 with the immersion liquid B, drying, and cutting to obtain a dry chemical test paper for sialidase detection.

[0042] In the above technical solution, in the immersion solution A, the concentration of the substrate is preferably 0.1-2 mg / mL.

[0043]In the above technical scheme, the pH of the buffer containing metal ions is preferably 3-9, more preferably 4-6, wherein the concentration of the buffer is preferably 0.1-1M...

Embodiment 1

[0060] Preparation of dry chemical test paper for sialidase detection: take 31ET filter paper, soak it evenly with soaking solution A, dry it in a high-temperature drying oven at 50°C for 25 minutes, and soak the filter paper evenly with soaking solution B at 60°C Dry in a high-temperature drying oven at ℃ for 15 minutes, dry, and cut to obtain dry chemical test paper for sialidase detection, which is sealed and stored at 2-8°C;

[0061] Immersion solution A: 0.1M potassium acetate buffer solution with pH 4.0, 0.5mg / mL 5-bromo-4-chloro-3-indole-α-D-N-acetylneuraminic acid salt, 0.1wt% zinc chloride, 10wt% % magnesium chloride, 5 wt% manganese chloride and 5 wt% Tween 20.

[0062] Infusion B: Absolute ethanol containing 0.5 mg / mL diazonium salt.

Embodiment 2

[0064] Preparation of dry chemical test paper for sialidase detection:

[0065] Take 7218 filter paper, soak it evenly with soaking solution A, dry it in a high-temperature drying oven at 80°C for 20 minutes, after drying, soak the filter paper evenly with soaking solution B, dry it in a high-temperature drying oven at 40°C for 25 minutes, and then dry it. Cut it out to get the dry chemical test paper for sialidase detection, and keep it sealed and stored at 2-8°C;

[0066] Immersion solution A: 0.5M potassium acetate buffer solution with pH 5.0, 0.1mg / mL 5-bromo-4-chloro-3-indole-α-D-N-acetylneuraminic acid salt, 15wt% zinc chloride, 0.1wt % magnesium chloride, 2.5 wt% manganese chloride and 10 wt% Tween 20.

[0067] Infusion B: Absolute ethanol containing 0.1 mg / mL diazonium salt.

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Abstract

The invention relates to dry chemical test paper for neuraminidase and a preparation method thereof, and belongs to the technical field of in-vitro diagnosis test paper. The dry chemical test paper solves the technical problem that in the prior art, a polyamine detection kit for detecting bacterial vaginosis is poor in specificity. The dry chemical test paper is filter paper with a substrate, a color developing agent, metal ions, a buffer and a surfactant dispersed, wherein the substrate is one or more of acetylneuraminic acid salt, an acetyl neuraminic acid salt derivative and 5-bromo-4-chloro-3-indolyl-alpha-D-N-acetylneuraminic acid salt, and the color developing agent is a diazonium salt. The test paper has the advantages of being high in accuracy, good in stability, simple to operateand suitable for popularization; and clinical data analysis results show that compared with an Amsel method, the chemical test paper has the advantages that the sensitivity is 89.5-90.0%, the specificity is 95.8-96%, the positive predictive value is 83.3-90.0%, the negative predictive value is 98.2-98.8%, the coincidence rate is 96.5-97%, the BV screening diagnosis requirement is basically met, and the diagnosis BV index is more objective.

Description

technical field [0001] The invention belongs to the technical field of in vitro diagnostic reagents, in particular to a sialidase dry chemical test paper and a preparation method thereof. Background technique [0002] Bacterial vaginosis (BV) is the most common vaginal infection in women of childbearing age, accounting for 40%-50% of vulvovaginal infections. In recent years, studies have found that bacterial vaginosis is not only associated with pelvic inflammatory disease, vaginitis, It is related to gynecological diseases such as infection, and can also cause intra-amniotic infection, premature rupture of membranes, puerperal infection, low birth weight, neonatal infection, and neonatal jaundice, which lead to adverse perinatal outcomes, so more and more people pay attention to it. [0003] The typical clinical symptom of this disease is that abnormal vaginal discharge increases significantly, which is thin homogeneous or thin paste, grayish white, grayish yellow or milky ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78G01N2021/7759
Inventor 孙德亚朱子是崔秀丽林月何浩会孙成艳
Owner DIRUI MEDICAL TECH CO LTD
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