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Method of extracting ginkgolide from gingko leaves

A technology of ginkgolide and ginkgo biloba, applied in the direction of organic chemistry and the like, can solve the problems of long time consumption, low extraction rate, complicated process and the like, and achieve the effects of reducing production cost, short process time and simple process.

Inactive Publication Date: 2018-12-18
HUBEI ENG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There are many extraction methods of existing ginkgolides, including solvent extraction method, column extraction method, solvent extraction-column extraction method, supercritical extraction method, subcritical water extraction method, and the existing solvent extraction method operation steps Many, the process is cumbersome, the extraction rate is low and the loss of solvent is relatively large
[0004] In the patent document with the application number CN200710050244.0, ethanol extracts of different concentrations were combined and filtered, and the filtrate was concentrated, then dissolved in ethanol with a volume concentration of 50%-60%, and crystallized to obtain high-purity Ginkgolides, the method for extracting ginkgolides, the yield is relatively low, because ginkgolides are soluble in ethanol, so it is very difficult to crystallize the total ginkgolides in ethanol solution, the resulting high-purity ginkgolides volume will be small
In the patent document with the application number CN201210595603, the process flow is relatively complex, time-consuming, costly, and yield low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The method that extracts the ginkgolide in the ginkgo leaf in the present embodiment is as follows:

[0023] (1) Weigh 100g of dried ginkgo leaves and crush them, put them into a container, add 300mL of ethanol with a volume fraction of 50%, heat in a water bath to 75°C, stir and extract for 20min, then press filter to obtain the filtrate, and repeat the extraction according to the above method 4 times, the filtrates were combined for 5 times and allowed to stand for 2 hours, and the precipitated insoluble matter was filtered to obtain an extract;

[0024] (2) Concentrate the extract under reduced pressure to obtain 100 mL of the concentrated extract;

[0025] (3) Add 100mL of ethyl acetate with a concentration of 99.5vol% to the concentrated extract, heat it in a water bath to 65°C, stir and extract for 20 minutes to separate the extract, repeat the above method 4 times, and combine 5 extractions solution, concentrated under reduced pressure to form an extract;

[00...

Embodiment 2

[0028] The method that extracts the ginkgolide in the ginkgo leaf in the present embodiment is as follows:

[0029] (1) Weigh 100g of dried ginkgo leaves and crush them, put them into a container, add 400mL of ethanol with a volume fraction of 80%, heat in a water bath to 70°C, stir and extract for 30min, then press filter to obtain the filtrate, and repeat the extraction according to the above method 5 times, the filtrates were combined for 6 times and allowed to stand for 4 hours, and the precipitated insoluble matter was filtered to obtain an extract;

[0030] (2) Concentrate the extract under reduced pressure to obtain 80 mL of the concentrated extract;

[0031] (3) Add 200mL of ethyl acetate with a concentration of 99.5vol% to the concentrated extract, heat it in a water bath to 70°C, stir and extract for 30 minutes to separate the extract, repeat the above method 5 times, and combine 6 extractions solution, concentrated under reduced pressure to form an extract;

[003...

Embodiment 3

[0034] The method that extracts the ginkgolide in the ginkgo leaf in the present embodiment is as follows:

[0035] (1) Weigh 100g of dried ginkgo leaves and grind them into a container, add 350mL of ethanol with a volume fraction of 99.5%, heat in a water bath to 72°C, stir and extract for 10min, then press filter to obtain the filtrate, and repeat the extraction according to the above method 5 times, the filtrates were combined for 6 times and allowed to stand for 3 hours, and the precipitated insoluble matter was filtered to obtain an extract;

[0036] (2) Concentrating the extract under reduced pressure to obtain 120 mL of the concentrated extract;

[0037] (3) Add 150mL of ethyl acetate with a concentration of 99.5vol% to the concentrated extract, heat it in a water bath to 68°C, stir and extract for 10 minutes to separate the extract, repeat the extraction 5 times according to the above method, and combine 6 times The extract is concentrated under reduced pressure to fo...

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Abstract

The invention relates to a method of extracting ginkgolide from gingko leaves, which includes steps of: drying and crushing the gingko leaves, extracting the gingko leaves with ethanol, recycling theethanol through pressure reduced concentration, extracting the gingko leaves with ethyl acetate and recycling the ethyl acetate, water-washing the extract, extracting the gingko leaves with petroleumether and recycling the petroleum ether to obtain a ginkgolide crude product. The method is simple in process, is short in time and low in cost, is high in yield and purity of the ginkgolide. The yield of the ginkgolide crude product is 0.25-1%, and purity of the same is 26-37%.

Description

technical field [0001] The invention relates to a method for extracting active ingredients from plants, in particular to a method for extracting ginkgolide from ginkgo leaves. Background technique [0002] Ginkgo biloba is a gymnosperm deciduous tree. There have been records of Ginkgo biloba used as medicine and diet since ancient times in my country. As a kind of natural medicine, Ginkgo biloba extract has various physiological activities and has been widely used for cardiovascular and cerebrovascular diseases. Improve the blood circulation of cardiovascular and cerebrovascular, because of its definite and significant medicinal effect, Ginkgo biloba extract has become the most famous single medicine in the world. The main components of Ginkgo biloba extract are flavonoids and terpene lactones. Terpene lactones mainly include ginkgolides A, B, C, J, M, K, L and bilobalide. The molecular structure of ginkgolides is complex , chemical synthesis and plant tissue culture are res...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D493/22
Inventor 况启生仇小艳姜益泉谢志兵唐本兴张杨杨新河史红安舒婷李安明李长春王立华田春元戴余军张福云
Owner HUBEI ENG UNIV
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