Construction method of near-infrared quantitative calibration model of royal jelly and detection method of royal jelly

A technology for calibrating models and constructing methods, which is applied in the field of infrared detection and can solve problems such as the content of 10-HDA in royal jelly

Active Publication Date: 2021-09-10
JILIN MODERN TRADITIONAL CHINESE MEDICINE ENG RES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the prior art, there is no relevant report on the rapid determination of 10-HDA content in royal jelly by using near-infrared spectroscopy

Method used

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  • Construction method of near-infrared quantitative calibration model of royal jelly and detection method of royal jelly
  • Construction method of near-infrared quantitative calibration model of royal jelly and detection method of royal jelly
  • Construction method of near-infrared quantitative calibration model of royal jelly and detection method of royal jelly

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] (1) Collect near-infrared spectrum of royal jelly samples: Take about 8g of royal jelly samples, put them in a 15ml centrifuge tube, put the near-infrared probe deep into the liquid surface, and ensure that the royal jelly completely wraps the probe, and use air as a reference to collect near-infrared spectra after deducting the background , to obtain the near-infrared spectrum of the sample.

[0054] (2) Determination of 10-HDA content in the sample by high-performance liquid chromatography: take 10-hydroxydecenoic acid reference substance, add methanol to make a solution containing 200 μg of 10-hydroxydecenoic acid per 1 ml, and obtain it. Take about 0.15g of royal jelly, weigh it accurately, put it in a 25ml measuring bottle, add about 20ml of methanol, shake it up, let it stand overnight, add methanol to the mark, shake it up, filter it, and take the filtrate to get it. The prepared sample was used to measure the content of 10-hydroxydecenoic acid in the royal jelly...

Embodiment 2

[0060] Use the established index model to randomly select samples that did not participate in the modeling for analysis, verify the consistency of the spectral acquisition parameter settings between the sample and the modeled sample, obtain the prediction results of each index, calculate the deviation between the predicted value and the measured value, and verify the accuracy of the model sex.

[0061]

[0062] Table 1 is used for the HPLC measured value of 10-HDA content in the royal jelly of model accuracy test and corresponding near-infrared model measured value (%)

[0063]

[0064] The NIR spectrum model shows that the 10-HDA content model in royal jelly, the internal verification straight line method is Y=0.9870X+0.019, the external verification straight line equation is Y=0.9506X+0.072, where X is the test value, Y is the predicted value, and the internal verification Coefficient of determination R 2 =0.9935, coefficient of determination of external verification ...

Embodiment 3

[0067] Embodiment 3 compares evaluation with original detection technology

[0068] The application and related consumables are also compared, see Table 2 for details.

[0069] Table 2 Comparison of detection technologies

[0070]

[0071] Note: * indicates significant difference compared with the traditional method (P# Compared with the traditional method, there was a very significant difference (P<0.01).

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Abstract

The invention relates to the field of infrared detection, in particular to a method for constructing a near-infrared quantitative calibration model of royal jelly and a detection method for royal jelly. The method is specifically as follows: select royal jelly with known 10-hydroxydecenoic acid content, and set it aside; collect royal jelly near-infrared spectral data; select spectral information under characteristic bands to correlate with the known 10-hydroxydecenoic acid content of royal jelly , using the partial least squares method and cross-validation method to establish a quantitative standard model; carry out near-infrared spectrum scanning on unknown royal jelly samples, and select the spectral information under the characteristic band, and import it into the established quantitative calibration model to obtain 10% of the unknown royal jelly samples. ‑Hydroxydecenoic acid content. Utilizing the near-infrared prediction model for the detection of 10-hydroxydecenoic acid content in royal jelly has the advantages of simple operation, fast analysis speed, and high accuracy of prediction results. The present invention provides an experimental basis for the determination and inspection of royal jelly quality, and has significant application value and market prospects.

Description

technical field [0001] The invention relates to the field of infrared detection, in particular to a method for constructing a near-infrared quantitative calibration model of royal jelly and a detection method for royal jelly. Background technique [0002] Royal jelly is a natural substance integrating nutrition, diet therapy and health care. It has antibacterial, anti-oxidation, anti-aging, lowering blood sugar, lowering blood fat, anti-tumor, regulating immunity, skin care and beauty and other physiological functions. It mainly contains protein, amino acid, organic acid and vitamin, and also a small amount of phytosterol, acetylcholine, adenosine phosphate and other ingredients. Royal jelly is rich in 10-hydroxydecenoic acid (10-HDA), which is not found in other substances, so it is called royal jelly acid. 10-HDA has become the focus of research in recent years and has become a symbolic component of royal jelly. At present, the most commonly used method for detecting 10-...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/359G01N21/3577G01N30/02
CPCG01N21/3577G01N21/359G01N30/02
Inventor 姜文月曲佳乐魏晓雨张新君王美慧边雨徐杰崔宪利胡铭
Owner JILIN MODERN TRADITIONAL CHINESE MEDICINE ENG RES CENT
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