Preparation method and application of fluorescent carbon dot for specifically identifying Cr<6+> and vitamin C

A technology of fluorescent carbon dots and vitamins, applied in chemical instruments and methods, fluorescence/phosphorescence, nano-carbon, etc., can solve the problem of only ingestion from food, achieve specific detection and rapid identification, easy operation, simple synthesis Effect

Active Publication Date: 2018-12-21
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows researchers to detect small amounts (<10 ppm) of certain substances within biological tissues quickly by measuring their ability to emit light when excited near visible wavelengths or other types of radiation. By combining these two techniques together it becomes easier than previous methods that required multiple steps like addition reagents beforehand. Additionally, this method could be used on live cell cultures where there may have been harmful environmental factors such as excessive UV exposure from sunlight during experimentation. Overall, this new technique simplifies sample preparations while improving accuracy over current analytical tools.

Problems solved by technology

This patented technical problem addressed in this patents relates to developing fast and sensitive methods or markers capable of detecting both metal ion contamination and hormone levels associated therewith without causing damage to humans' healthy cells like bacteria or plants.

Method used

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  • Preparation method and application of fluorescent carbon dot for specifically identifying Cr&lt;6+&gt; and vitamin C
  • Preparation method and application of fluorescent carbon dot for specifically identifying Cr&lt;6+&gt; and vitamin C
  • Preparation method and application of fluorescent carbon dot for specifically identifying Cr&lt;6+&gt; and vitamin C

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Carbon dot synthesis, the steps are as follows:

[0043] Dissolve 2.4g of anhydrous citric acid in 16mL of ultrapure water, then add 800µL of N,N-dimethylaniline liquid, and heat the mixture to 160 o C, the reaction was continued for 10 hours, and the reaction solution was ultra-filtered and separated by column chromatography to obtain the carbon dot.

Embodiment 2

[0045] Carbon dot synthesis, the steps are as follows:

[0046] Dissolve 0.6g of anhydrous citric acid in 16mL of ultrapure water, then add 800µL of N,N-dimethylaniline liquid, and heat the mixture to 160 o C, the reaction was continued for 10 hours, and the reaction solution was ultra-filtered and separated by column chromatography to obtain the carbon dot.

Embodiment 3

[0048] Carbon dot synthesis, the steps are as follows:

[0049] Dissolve 4.8g of anhydrous citric acid in 16mL of ultrapure water, then add 800µL of N,N-dimethylaniline liquid, and heat the mixture to 160 o C, the reaction was continued for 10 hours, and the reaction solution was ultra-filtered and separated by column chromatography to obtain the carbon dot.

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Abstract

The invention provides a preparation method and application of a fluorescent carbon dot for specifically identifying Cr<6+> and vitamin C. The method comprises the following steps: adding anhydrous citric acid into water and dissolving anhydrous citric acid, adding N,N'-dimethylaniline after dissolving, transferring mixed liquid into a microwave tube, heating and reacting through microwave, carrying out ultrafiltration of reaction liquid and carrying out column chromatographic separation of the reaction liquid to obtain the carbon dot. The fluorescent carbon dot as a probe is extremely simpleto synthesize and convenient to operate, is capable of achieving specific detection and rapid identification of Cr<6+> and vitamin C, and is also capable of achieving detection of endogenous and exogenous Cr<6+> and vitamin C in living cells.

Description

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Claims

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Application Information

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Owner ZHENGZHOU UNIV
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