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Test strip for detecting pendimethalin, and preparation method and application thereof

A pendimethalin and test strip technology, applied in the detection field of pendimethalin, can solve the problems of inability to realize rapid detection of large-scale samples and high equipment dependence, and achieve short detection time, high sensitivity and specificity. strong effect

Active Publication Date: 2018-12-21
ZHENGZHOU TOBACCO RES INST OF CNTC +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the problem that existing methods for detecting pendimethalin are highly dependent on equipment and cannot realize rapid detection of large batches of samples, and provide a method with simple operation, high sensitivity, fast detection speed and low cost. Test strips with low cost and not limited by detection equipment and their preparation methods and applications, so as to realize rapid detection and on-site monitoring of pendimethalin in large batches of samples

Method used

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  • Test strip for detecting pendimethalin, and preparation method and application thereof
  • Test strip for detecting pendimethalin, and preparation method and application thereof
  • Test strip for detecting pendimethalin, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Preparation of test strips for detecting pendimethalin

[0044] The preparation method of this test strip mainly comprises the following steps:

[0045] 1) Prepare a conjugate release pad sprayed with pendimethalin monoclonal antibody-colloidal gold marker;

[0046] 2) Prepare a reaction membrane with a detection line coated with pendimethalin hapten-carrier protein conjugate and a quality control line coated with goat anti-mouse anti-antibody;

[0047] 3) Assemble the conjugate release pad prepared in 1) and 2), the reaction membrane, the sample absorption pad, the water absorption pad and the PVC bottom plate into a test strip.

[0048] The following step-by-step detailed description:

[0049] 1. Synthesis of pendimethalin hapten (see the synthetic route image 3 ) and identification

[0050] Take 1.00 g of N-(1-ethylpropyl)-3,4-dimethylaniline, add 80 mL of acetone to dissolve, add 0.35 g of potassium hydroxide, add 1.21 g of ethyl 4-bromobutyrate, and ...

Embodiment 2

[0093] Example 2 Detection of Pendimethalin in Tobacco Leaf Samples

[0094] 1. Pretreatment of samples

[0095] Weigh 1.0±0.05 g crushed sample into a polystyrene centrifuge tube, add 4 mL of methanol, vortex for 3 min, centrifuge at 6000 rpm for 5 min at room temperature (20-25 °C), take 25 μL supernatant and add 475 μL Mixed with deionized water before testing.

[0096] 2. Test with test strips

[0097] Use a disposable straw to absorb 2-3 drops of the sample solution to be tested and drop it vertically into the sample injection hole. When the liquid flows, start timing, react for 10 minutes, and judge the result.

[0098] 3. Analyze test results

[0099] Negative (-): The color of the T line is darker than that of the C line or consistent with the color of the C line, indicating that the concentration of pendimethalin in the sample is lower than the detection limit, such as figure 2 a, 2b.

[0100] Positive (+): The T-line color is lighter than the C-line color or the...

Embodiment 3

[0102] Embodiment 3 sample detection example

[0103] 1. Detection limit test

[0104] Take blank cured tobacco leaf samples, add pendimethalin to the final concentrations of 2.5, 5, and 10 mg / kg respectively, take test strips for detection, and repeat the measurement three times for each sample.

[0105] When testing the cured tobacco leaf sample with a test strip, when the pendimethalin concentration is 2.5 mg / kg, the test strip shows that the color of the T line is darker than that of the C line or consistent with the color of the C line. Negative; when the added concentration of pendimethalin is 5 or 10mg / kg, the test strip shows that the T line color is lighter than the C line color or the T line does not develop color, which is positive. It shows that the detection limit of this test strip for pendimethalin in cured tobacco leaves is 5 mg / kg.

[0106] 2. False positive rate and false negative rate test

[0107] Take 20 positive samples of cured tobacco leaves with kno...

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Abstract

The invention provides atest strip for detecting pendimethalin, and a preparation method and application thereof. The test strip comprises a sample absorbing cushion, a conjugate releasing cushion, areacting film, a water absorbing cushion and a base plate and is characterized in that the reacting film is provided with detection lines coated with pendimethalin hatpin-carrier protein couplers andquality control lines coated with anti-goat and anti-mouse anti-bodies, and pendimethalin monoclonal antibody-colloid gold markers are sprayed on the conjugate releasing cushion. The pendimethalin hatpin is prepared through the steps that N-(1-ethyl propyl ether)-3,4-methyl toluidine reacts with 4-ethyl 4-bromobutyrate to generate hydrocarbylation N-(1-ethyl propyl ether)-3,4-methyl toluidine, then the hydrocarbylation N-(1-ethyl propyl ether)-3,4-methyl toluidine reacts with concentrated nitric acid to generate nitro hydrocarbonylation N-(1-ethyl propyl ether)-3,4-methyl toluidine, and then the nitro hydrocarbonylation N-(1-ethyl propyl ether)-3,4-methyl toluidine reacts with KOH. The test striphas the advantages of being easy and convenient to operate, high in sensitivity, high in detecting speed, low in cost, and not limited by detecting equipment and can conduct rapid detection and on-site monitoring on great batches of pendimethalin samples.

Description

technical field [0001] The invention relates to the detection of pendimethalin, in particular to a test strip for detecting pendimethalin and a preparation method and application thereof, which are particularly suitable for detection of pendimethalin residues in tobacco leaves. Background technique [0002] Pendimethalin belongs to dinitroaniline herbicides, and its mechanism of action is to inhibit meristem cell division. The part of pendimethalin absorbed by dicotyledons is hypocotyl, and the part absorbed by monocots is sprout. Pendimethalin is suitable for corn, soybean, wheat, peanut, cotton and other dryland crops to control annual grass and broad-leaved weeds such as crabgrass, foxtail and purslane. Tobacco is mainly used for chemical bud suppression and tobacco weed control. According to the classification standard of acute toxicity of pesticides in China, pendimethalin is classified as a low toxicity compound. According to the toxic classification standard of the...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/58G01N33/577G01N33/531
CPCG01N33/531G01N33/558G01N33/577G01N33/58
Inventor 范子彦陈黎何方洋李旭刘惠民唐纲岭潘立宁颜权平鲁亚辉
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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