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A time-resolved fluorescent immunochromatographic test strip for detecting iprodione and its preparation method and application

A technology of time-resolved fluorescence and immunochromatography test paper, which is applied in the fields of analytical materials, biological testing, fluorescence/phosphorescence, etc.

Active Publication Date: 2021-07-06
ZHENGZHOU TOBACCO RES INST OF CNTC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently there is no time-resolved fluorescent immunochromatographic test strip for the detection of iprodione in tobacco and tobacco products

Method used

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  • A time-resolved fluorescent immunochromatographic test strip for detecting iprodione and its preparation method and application
  • A time-resolved fluorescent immunochromatographic test strip for detecting iprodione and its preparation method and application
  • A time-resolved fluorescent immunochromatographic test strip for detecting iprodione and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 The composition of the time-resolved fluorescent immunochromatographic test strip for detecting iprodione

[0045] 1. Test strips

[0046] see figure 1 : The test strip is composed of a bottom plate, a sample absorbent pad, a conjugate release pad, a nitrocellulose membrane and an absorbent pad;

[0047] The sample absorbent pad 1, the conjugate release pad 2, the nitrocellulose membrane 3 and the water absorbent pad 4 are overlapped and pasted on the bottom plate 7 in sequence, and 1 / 3 of the conjugate release pad is covered by the sample absorbent pad from the starting end , the end of the conjugate release pad is connected to the beginning of the nitrocellulose membrane, the end of the nitrocellulose membrane is connected to the beginning of the absorbent pad, the beginning of the sample absorbent pad is aligned with the beginning of the PVC bottom plate, and the end of the absorbent pad is connected to the end of the PVC bottom plate alignment;

[0048]...

Embodiment 2

[0050] Example 2 Preparation of time-resolved fluorescent immunochromatographic test strips for detecting iprodione

[0051] The preparation method of the time-resolved fluorescent immunochromatography test strip for detecting iprodione mainly comprises the following steps:

[0052] 1) Preparation of the conjugate release pad: label the iprodione monoclonal antibody with commercially available fluorescent microspheres, and dilute it with a specific buffer system, soak the conjugate release pad in the dilution buffer, and vacuum freeze-dry Post-preparation;

[0053] 2) Preparation of nitrocellulose membrane: Spray the iprodione hapten-carrier protein conjugate onto the detection area on the nitrocellulose membrane to make the detection area; spray goat anti-mouse anti-antibody onto the nitrocellulose membrane The scope of the quality control area above is made into the quality control area;

[0054] 3) Assembling and cutting: Paste the sample absorption pad, the conjugate rel...

Embodiment 3

[0118] Example 3 Application of time-resolved fluorescent immunochromatographic test strips for detecting iprodione

[0119] 1. Tobacco sample pretreatment

[0120] Weigh 1.0±0.05 g of crushed sample to be tested into a 50 mL centrifuge tube, add 10 mL of 50% methanol aqueous solution, vortex for 1 min, and centrifuge at 3000 rpm for 5 min; take 100 μL of supernatant, add 400 μL of sample Reconstituted solution, mixed to be tested.

[0121]2. Test with test strips

[0122] Draw 100 μL of the sample solution to be tested and drop it vertically into the sample hole of the test paper card. When the liquid flows, start timing and react for 10 minutes; insert the test paper card into the carrier of the KFT-100A fluorescence detector, and select the sample to be Check the items, press the "Start Test" button, the fluorescence detector will automatically scan the test paper card, and read or print the test results on the display screen of the instrument.

[0123] 3. Analysis of te...

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Abstract

The invention discloses a time-resolved fluorescent immunochromatographic test strip for detecting iprodione, a preparation method and application thereof. The test strip includes a bottom plate and a sample absorption pad, a conjugate release pad, a nitrocellulose membrane, and a water absorption pad that are sequentially overlapped and pasted on the bottom plate. The conjugate release pad is embedded with iprodione monoclonal antibody labeled with fluorescent microspheres. , a detection area and a quality control area are fixed on the nitrocellulose membrane, the detection area is sprayed with iprodione hapten-carrier protein conjugate, the quality control area is sprayed with goat anti-mouse anti-antibody, and the iprodione hapten is composed of 3 , 5-dichlorophenyl isocyanate and glycine ethyl ester hydrochloride, 6-aminocaproic acid methyl ester hydrochloride, etc. are obtained through a series of reactions. The test strip and the detection method provided by the invention have the advantages of simple operation, high sensitivity, fast detection speed and low cost, and can realize rapid detection of iprodione in a large number of samples.

Description

technical field [0001] The invention belongs to the field of pesticide residue detection, and in particular relates to a time-resolved fluorescent immunochromatographic test strip for detecting iprodione in tobacco and tobacco products, a preparation method and application thereof. Background technique [0002] Iprodione belongs to dicarboximides, and is a broad-spectrum contact-type protective fungicide, widely used in tobacco, fruit tree, vegetable disease control and fruit storage and preservation. Iprodione can be absorbed systemically through roots, and can effectively control fungi that are resistant to benzimidazole systemic fungicides. Its main control targets are diseases caused by Botrytis, Alternaria, and Sclerotinia, such as Botrytis cinerea, early blight, black spot, and Sclerotinia. For different crops, my country has formulated the maximum residue limit standard of iprodione, among which the maximum residue limit in rapeseed and cucumber is 2 mg / kg, and the m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/558G01N33/58G01N33/533G01N21/64
CPCG01N21/6408G01N33/533G01N33/558G01N33/582G01N33/585
Inventor 陈黎范子彦刘惠民唐纲岭崔华鹏樊美娟赵乐王晓瑜秦亚琼王洪波
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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