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A nano-hydrogel mediated by bone marrow mesenchymal stem cells and its preparation and application

A nano-hydrogel, bone marrow mesenchymal technology, applied in the preparations, pharmaceutical formulations, emulsion delivery and other directions for in vivo experiments, can solve the problems of insufficient targeting ability of contrast agents, inaccurate imaging, etc., and achieve good immunity. Escape and biocompatibility, good MR imaging effect, good water solubility

Active Publication Date: 2021-07-02
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide a nano-hydrogel mediated by bone marrow mesenchymal stem cells and its preparation method and application, so as to overcome the defects of insufficient targeting ability of contrast agents and inaccurate imaging in the prior art

Method used

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  • A nano-hydrogel mediated by bone marrow mesenchymal stem cells and its preparation and application
  • A nano-hydrogel mediated by bone marrow mesenchymal stem cells and its preparation and application
  • A nano-hydrogel mediated by bone marrow mesenchymal stem cells and its preparation and application

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Embodiment 1

[0060] (1) Dissolve 1.09g of ferric chloride hexahydrate in 40mL of diethylene glycol (also known as diethylene glycol, DEG), and then 0.47g of sodium citrate (Na 3 Cit) was dissolved in the above solution, and stirred at 80° C. for 1 hour under an air atmosphere. After the sodium citrate was completely dissolved, 1.312 g of anhydrous sodium acetate was added to the above solution, and the stirring was continued until the sodium acetate powder was completely dissolved, and then Transfer the solution to a 50mL autoclave, and react at 200°C for 4 hours; after the reaction, cool to room temperature naturally, transfer the product to a 50mL centrifuge tube and centrifuge at 8500rpm for 15 minutes, discard the supernatant, and return to the solution with absolute ethanol. Dissolve, centrifuge at 8500rpm for 15 minutes, repeat the operation 3 times, and then dry the precipitate at 60°C to obtain ultra-small ferric oxide nanoparticles, that is, ultra-small iron oxide nanoparticles sta...

Embodiment 2

[0066] Get the Fe prepared by embodiment 1 respectively 3 o 4 , PEI-Fe 3 o 4 Nanoparticles and AG / PEI-Fe 3 o 4 Nanogel 2mg was dissolved in ultrapure water, ultrasonically uniform, measured surface potential and hydrated particle size, as shown in Table 1, the prepared Fe 3 o 4 , PEI-Fe 3 o 4 and AG / PEI-Fe 3 o 4 The potentials are -34.5mV, +42.6mV, -18.7mV respectively; the hydrated particle sizes are 26.3nm, 115.8nm and 216.2nm respectively. From the experimental results, it can be concluded that the surface potential of ultra-small iron oxide nanoparticles increases after PEI is modified, and the hydrodynamic diameter increases, which is mainly caused by the surface modification of PEI. Zeta potential measurement results show that AG / PEI-Fe 3 o 4 The surface potential of the nanogel is -18.7mV, and the crosslinker PEI-Fe 3 o 4 The surface potential of +42.6mV proves that AG and PEI-Fe 3 o 4 successful crosslinking. Its hydrodynamic diameter is 216.2nm, and t...

Embodiment 3

[0070] Take by weighing three kinds of materials that embodiment 1 prepares respectively: Fe 3 o 4 , PEI-Fe 3 o 4 and AG / PEI-Fe 3 o 4 Perform thermogravimetric analysis (e.g. Figure 4 shown) and infrared spectroscopy (such as Figure 5 shown). TGA test results showed that Fe 3 o 4 The weight loss is 74.8% (see Figure 4 a), Fe 3 o 4 -PEI and AG / PEI-Fe 3 o 4 The weight loss was 56.2% (see Figure 4 b) and 31.7% (see Figure 4 middle c), thus quantitatively analyzing the AG / PEI-Fe 3 o 4 The contents of PEI and AG in the nanogels were 18.6% and 24.5%, respectively. Infrared test shows 3397cm in a, b, c -1 The peak at is the stretching vibration peak of OH on the adsorbed water molecule, 2926cm -1 and 2855cm -1 The nearby characteristic absorption peaks are attributed to the stretching vibration of methylene on sodium citrate, and at 1396-1642cm -1 (Stretching vibration of C=O) and 1028-1075cm -1 (C-O-C stretching vibration) peaks, all in Fe 3 o 4 , Fe 3 o ...

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Abstract

The invention relates to a nano hydrogel mediated by bone marrow mesenchymal stem cells and its preparation and application. The nano hydrogel is ultra-small ferric oxide modified by sodium alginate and polyethyleneimine. The preparation method comprises: the preparation of sodium citrate stabilized ultra-small ferric oxide nanoparticles, Fe 3 O 4 ‑PEI aqueous solution preparation, W / O / W polymer emulsion preparation, ultra-small Fe3O4-loaded hybrid sodium alginate nanohydrogel preparation, bone marrow mesenchymal stem cell-mediated nanohydrogel preparation. The method has simple process, mild reaction conditions, easy operation and low cost.

Description

technical field [0001] The invention belongs to the field of magnetic resonance imaging (MRI) contrast agent and its preparation and application, and in particular relates to a nano hydrogel mediated by bone marrow mesenchymal stem cells and its preparation method and application. Background technique [0002] Malignant tumors have always been the number one killer of human life, with the characteristics of high mortality, difficult treatment and rapid deterioration. Therefore, early diagnosis and specific treatment of tumors are particularly important. Currently, tumor detection methods mainly include ultrasound imaging, CT imaging, nuclear medicine (PET or SPECT) imaging, and magnetic resonance imaging (MRI). With the development of magnetic resonance technology, the scanning time is gradually shortened, the resolution is gradually improved, and the detection of small lesions is more accurate, which also makes magnetic resonance imaging technology a new disease detection ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/18A61K49/08A61K49/12
CPCA61K49/08A61K49/124A61K49/1803
Inventor 曹雪雁史向阳郝欣欣徐琲王小萌陈环
Owner DONGHUA UNIV
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