A method for increasing the yield of chlorella polysaccharide
A technology for chlorella and algal polysaccharide is applied in the field of increasing the output of chlorella polysaccharide, can solve the problems of low sugar production efficiency, yield, purity, complex process, etc., and achieves the advantages of promoting industrialized production, simplifying processing operations and low production cost. Effect
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Embodiment 1
[0028] A method for improving the production of chlorella polysaccharides, comprising the steps of:
[0029] The Chlorella vulgaris liquid in the logarithmic growth phase was inoculated into the reaction tank containing the culture solution according to the inoculation amount of 5%, the light intensity was 5000lux, cultured at 22°C, the light-to-dark ratio was 18:6, and the ventilation rate was 0.2vvm The components of the culture solution are: glucose 5g / L, potassium nitrate 2g / L, sodium chloride 1g / L, borax 0.5g / L, potassium dihydrogen phosphate 0.5g / L, calcium carbonate 0.1g / L, Magnesium sulfate heptahydrate 50mg / L, ferric ammonium citrate 20mg / L, sodium naphthalene acetate 10mg / L, gibberellin 10mg / L;
[0030] After cultivating for 72 hours, add glucose and arachidonic acid so that the concentration of glucose in the culture solution is 3g / L and the concentration of arachidonic acid is 100mg / L, continue to cultivate for 48 hours, and centrifuge the culture solution to colle...
Embodiment 2
[0032] A method for improving the production of chlorella polysaccharides, comprising the steps of:
[0033] The Chlorella vulgaris liquid in the logarithmic growth phase was inoculated into the reaction tank containing the culture solution according to the inoculation amount of 7%, the light intensity was 6000lux, cultured at 23°C, the light-to-dark ratio was 18:6, and the ventilation rate was 0.3vvm The components of the culture solution are: glucose 5g / L, potassium nitrate 2g / L, sodium chloride 1g / L, borax 0.5g / L, potassium dihydrogen phosphate 0.5g / L, calcium carbonate 0.1g / L, Magnesium sulfate heptahydrate 50mg / L, ferric ammonium citrate 20mg / L, sodium naphthalene acetate 10mg / L, gibberellin 10mg / L;
[0034] After cultivating for 72 hours, add glucose and arachidonic acid so that the concentration of glucose in the culture solution is 4g / L and the concentration of arachidonic acid is 200mg / L, continue to cultivate for 72 hours, and centrifuge the culture solution to colle...
Embodiment 3
[0036] A method for improving the production of chlorella polysaccharides, comprising the steps of:
[0037] The Chlorella vulgaris liquid in the logarithmic growth phase was inoculated into the reaction tank containing the culture solution according to the inoculation amount of 6%, the light intensity was 5500lux, cultured at 24°C, the light-dark ratio was 18:6, and the ventilation rate was 0.3vvm The components of the culture solution are: glucose 5g / L, potassium nitrate 2g / L, sodium chloride 1g / L, borax 0.5g / L, potassium dihydrogen phosphate 0.5g / L, calcium carbonate 0.1g / L, Magnesium sulfate heptahydrate 50mg / L, ferric ammonium citrate 20mg / L, sodium naphthalene acetate 10mg / L, gibberellin 10mg / L;
[0038] After cultivating for 72 hours, add glucose and arachidonic acid so that the concentration of glucose in the culture solution is 3g / L and the concentration of arachidonic acid is 200mg / L, continue to cultivate for 48 hours, and centrifuge the culture solution to collect ...
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