Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Pichia guilliermondii bacterium liquid biocontrol microbial agent and method for preparing same

A technology of biocontrol agents and yeasts, applied in biochemical equipment and methods, methods based on microorganisms, and yeast-containing food ingredients, etc., can solve the problems of ineffective control and few reports, and achieve good fruit biocontrol Effect, high survival rate, effect of maintaining potency

Inactive Publication Date: 2019-01-04
JIANGSU UNIV
View PDF3 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are not many reports of antagonistic bacteria that have significant control effects on anthracnose diseases such as loquat and nectarine, and the control effect is not obvious.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Pichia guilliermondii bacterium liquid biocontrol microbial agent and method for preparing same
  • Pichia guilliermondii bacterium liquid biocontrol microbial agent and method for preparing same
  • Pichia guilliermondii bacterium liquid biocontrol microbial agent and method for preparing same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: Isolation and identification of bacterial strain

[0024] Pichia pastoris Y35-1 ( Pichia guilliermondii Y35-1) was screened by our laboratory, and its antibacterial effect has been verified to be very good. It has been preserved in China Center for Type Culture Collection (Address: Wuhan, China, Wuhan University; deposit number is CCTCC NO: M2016719) on December 5, 2016.

[0025] The main steps of strain screening and identification process are as follows:

[0026] Isolation of antagonistic yeast: collect mature and healthy loquat fruit from the agricultural ecological orchard of Gaoqiao Town, Zhenjiang City, isolate and screen the strains on the surface of the intact fruit, select yeast single colonies with different culture characteristics and separate and purify them on the NYDA plate, pick each single Colonies were cryopreserved.

[0027] Preparation of antagonistic yeast suspension: resuspend the cultured yeast to form a suspension, calculate the ...

Embodiment 2

[0037] Embodiment 2: the preparation of Pichia mongolia liquid biocontrol agent

[0038] First, carry out enrichment culture of yeast: first thaw the deep-frozen Pichia mongolica in a water bath, insert it into NYDB liquid medium, culture it in a shaker flask at 28°C and 200r / min for 24-48h, repeat the culture once, and carry out the yeast Bacteria activation. Then, two loops of the activated Pichia monteritima were picked and inoculated in NYDB seed medium (50mL / 250mL), and cultured at 28°C and 200r / min for 24 hours; then 100μL of the obtained seed culture medium was taken Insert into NYTB fermentation medium (50mL / 250mL), culture at 28°C, 200r / min for 48 hours; NYDB medium includes 8g of beef extract, 5g of yeast extract, 10g of glucose, 1000mL of distilled water, natural pH; NYTB medium includes beef extract 8g, yeast extract 5g, trehalose 10g, distilled water 1000mL, natural pH.

[0039] Place the enriched and cultured Pichia montaglis fermentation broth in a centrifuge ...

Embodiment 3

[0041] Embodiment 3: the influence of each added component of Pichia mongolia liquid biocontrol agent on the effect

[0042] In this embodiment, the effect of different addition amounts of each component has been verified, and the specific method is as follows:

[0043] Single-factor screening of liquid preparations with protective agents: the protection results of each added protective agent are shown in Table 1. When the concentration of trehalose was 5 wt %, the survival of Y35-1 yeast cells reached the highest value, which was 75.6%; the concentration of trehalose was 2.5 wt % % and 10 wt %, the survival rates were 66.5% and 62.0%, respectively, so 5 wt % trehalose was selected as the optimal addition amount.

[0044] Glutathione plays a protective role in reduction and stress resistance, and the yeast survival rates at 0.01 wt %, 0.02 wt %, and 0.03 wt % concentrations were 63.6%, 78.5%, and 65.5%, respectively, so 0.02 wt % glutathione was selected Glutathione is the op...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a pichia guilliermondii bacterium liquid biocontrol microbial agent and a method for preparing the same, and belongs to the technical field of biocontrol microbial agent preparation. The pichia guilliermondii bacterium liquid biocontrol microbial agent in a formula comprises 1*10<6>-1*10<9> CFU / mL of pichia guilliermondii, 2.5-7.5 wt% of trehalose, 0.01-0.03 wt% of glutathione, 0.01-0.03 wt% of ascorbic acid and 0.02-0.05 mol / L of phosphoric acid buffer solution with the pH (potential of hydrogen) of 6.5. The pichia guilliermondii bacterium liquid biocontrol microbial agent and the method have the advantages that fruit biocontrol effects of the pichia guilliermondii bacterium liquid biocontrol microbial agent are further verified, and as shown by results, the fruitincidence and the diameters of fruit disease speckles of nectarine treated by the Y35-1 pichia guilliermondii bacterium liquid biocontrol microbial agent are obviously reduced as compared with the fruit incidence and the diameters of fruit disease speckles of comparison groups, and are significantly different from the fruit incidence and the diameters of fruit disease speckles of the comparison groups, and occurrence and development of anthranose in nectarine and loquat fruits can be effectively inhibited by the pichia guilliermondii bacterium liquid biocontrol microbial agent which is a liquid preparation prepared by the aid of the method.

Description

technical field [0001] The present invention relates to a kind of Pichia mongolica ( Pichia guilliermondii ) Liquid biocontrol agent and preparation method thereof, belonging to the technical field of biocontrol agent preparation. Background technique [0002] Nectarines and loquats, as one of the widely cultivated fruits from my country, have a moderately sweet and sour taste and high nutritional value. However, due to the thin skin of nectarines and loquats, ripening and picking in the early summer season with high temperature and humidity, they are extremely vulnerable to mechanical damage and microbial attack during picking, transportation and storage, and rot, resulting in huge economic losses. [0003] Anthracnose is one of the main diseases that often occur in the post-harvest transportation and storage of various fruits. Colletotrichum gloeosporioides ) and Anthracnose oxysporum ( Colletotrichum acutatum ) two kinds. For a long time, the control of fruit dise...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/16A23B7/155C12R1/84
CPCA23B7/155A23V2002/00C12N1/16C12N1/165C12R2001/84A23V2200/10A23V2250/76
Inventor 孙卫红周秋阳杨慧慧王玲郑鸿儒温新宇
Owner JIANGSU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com