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Stem cell culture perfusion device

A technology of stem cell culture and perfusion, which is applied in the field of biological culture, can solve the problems of cells losing their ability to clone and differentiate, and the degree of cell expansion is limited, and achieve the effects of high reliability, simple operation, and strong practicability

Inactive Publication Date: 2019-01-22
南京至泰生物医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional cell maintenance and expansion rely on two-dimensional (2-D) culture techniques using plastic culture plates and heterogeneous media. Under this method, cell expansion is limited, and after long-term passage, cells often lose their ability to clone and differentiate

Method used

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  • Stem cell culture perfusion device
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Embodiment Construction

[0013] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0014] exist figure 1 In the shown embodiment, the present invention includes an injection tube 1, a perfusion bottle 2, a perfusion mechanism 3, a blowout prevention tube 4, a cleaning mechanism 5, a pipe joint 6, a casing head 7, an air pipe 8, a sewage pipe 9, and a casing 10. Oil pipe 11, gas injection joint 12, connector 13, nozzle 14, coiled tubing channel 15, and inner annular space channel 16.

[0015] Including the perfusion bottle 2, the perfusion mechanism 3, and the cleaning mechanism 5, the perfusion mechanism 3 is installed under the perfusion bottle 2, the blowout prevention pipe 4 is installed under the perfusion mechanism 3, the cleaning mechanism 5 is installed under the blowout prevention pipe 4, and the cleaning mechanism 5 is installed There is a pipe joint 6, the pipe joint 6 is connected to the ventilation pipe 8, and a casing he...

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PUM

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Abstract

The invention provides a stem cell culture perfusion device. The stem cell culture perfusion device comprises a flow injecting tube, a perfusion bottle, a perfusion mechanism, a blow-out-preventing pipe, a cleaning mechanism, a pipe joint, a sleeve head, a breather pipe, a blow-off pipe, a sleeve, an oil tube, an air injecting connector, a connector, a spray head, a continuous pipe channel and aninner ring hollow channel. The stem cell culture perfusion device is strong in practicability, easy to operate, good in safety, high in cleanliness, convenient to use and high in reliability.

Description

technical field [0001] The invention relates to a device in the field of biological culture, in particular to a stem cell culture perfusion device which improves the culture efficiency. Background technique [0002] Stem cells (SCs) hold great promise in cell therapy, tissue engineering and regenerative medicine, as well as pharmaceutical and biotechnological applications. Stem cells have the ability to self-renew and differentiate into specific cell types (depending on the source from which the stem cells were isolated). However, the clinical application of stem cells requires the use of high-quality and high-number stem cells, which requires large-scale expansion of stem cells, and then efficient and uniform differentiation into functional derivatives. Traditional cell maintenance and expansion rely on two-dimensional (2-D) culture techniques using plastic culture plates and heterogeneous media. Under this method, cell expansion is limited, and after long-term passage, ce...

Claims

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Application Information

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IPC IPC(8): C12M3/00
CPCC12M29/06C12M29/10
Inventor 万源王礼学王国相
Owner 南京至泰生物医药科技有限公司