Preparation method of formamide phenazine bio-fungicide

A biological fungicide, the technology of caramidoxazine, which is applied in the field of microorganisms, can solve the problems of low yield of carboxyzine, complex fermentation operation, high overall cost, etc., and achieve the effect of good effect, short fermentation cycle and low cost

Active Publication Date: 2019-01-22
JIANGXI POROR CROPS ENG
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the yield of caramidozine prepared by the existing fermentation culture method is lo

Method used

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  • Preparation method of formamide phenazine bio-fungicide
  • Preparation method of formamide phenazine bio-fungicide

Examples

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Example Embodiment

[0038] The present invention provides a preparation method of carboxamidophenazine biological fungicide, including the following steps:

[0039] S1. Inoculate the activated Pseudomonas chlorophylla into liquid medium A, shake flask culture, and obtain seed liquid A. Among them, the liquid medium A includes the following components by weight: 1-5 parts of glucose, 1-5 parts of tryptone, 0.5-1.5 parts of ethanol, 0.5-1 parts of acetone, 0.05-0.15 parts of potassium dihydrogen phosphate and chlorinated 0.05-0.1 parts of sodium, 100-120 parts of water, pH 6.5-7.3. The conditions of shaking flask culture are: shaking culture in a shaker at 25-30°C for 15-20 hours, and the rotating speed of the shaker is 200-250r / min.

[0040] S2. Inoculate the activated Pseudomonas aeruginosa into liquid medium B, shake flask culture, and obtain seed liquid B. Wherein, the liquid medium B includes the following components by weight: 0.5-3 parts of sucrose, 0.5-2 parts of glucose, 2-5 parts of tryptone...

Example Embodiment

[0048] Example 1

[0049] A preparation method of carboxamidophenolazine biological fungicide, including the following steps:

[0050] S1. Inoculate the activated Pseudomonas chlorophylla into liquid medium A, shake flask culture, and obtain seed liquid A. Wherein, the liquid medium A includes the following parts by weight: 3 parts glucose, 3 parts tryptone, 1 part ethanol, 0.6 parts acetone, 0.1 parts potassium dihydrogen phosphate and 0.07 parts sodium chloride, 110 parts water, pH7. 0. The conditions of shaking flask culture are: shaking culture in a shaker at 27°C for 18 hours, and the rotating speed of the shaker is 220r / min.

[0051] S2. Inoculate the activated Pseudomonas aeruginosa into liquid medium B, shake flask culture, and obtain seed liquid B. Among them, the liquid medium B includes the following components by weight: 2 parts of sucrose, 1 part of glucose, 4 parts of tryptone, 1.7 parts of glycerol, 0.1 part of potassium dihydrogen phosphate, 0.06 part of magnesium ...

Example Embodiment

[0059] Example 2

[0060] A preparation method of carboxamidophenolazine biological fungicide, including the following steps:

[0061] S1. Inoculate the activated Pseudomonas chlorophylla into liquid medium A, shake flask culture, and obtain seed liquid A. Wherein, the liquid medium A includes the following components by weight: 1 part glucose, 1 part tryptone, 0.5 parts ethanol, 0.5 parts acetone, 0.05 parts potassium dihydrogen phosphate and 0.05 parts sodium chloride, 100 parts water, pH 6. 5. The conditions of shaking flask culture are: shaking culture in a shaker at 25° C. for 20 hours, and the rotating speed of the shaker is 200 r / min.

[0062] S2. Inoculate the activated Pseudomonas aeruginosa into liquid medium B, shake flask culture, and obtain seed liquid B. Among them, the liquid medium B includes the following components by weight: 0.5 parts of sucrose, 0.5 parts of glucose, 2 parts of tryptone, 1.5 parts of glycerol, 0.05 parts of potassium dihydrogen phosphate, 0.05 ...

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Abstract

The invention provides a preparation method of a formamide phenazine bio-fungicide. The preparation method comprises the following steps: firstly, implementing shake-flask culture on activated pseudomonas chlororaphis and pseudomonas aeruginosa, so as to obtain a seed solution A and a seed solution B; inoculating a fermentation medium with the seed solution A, conducting fermentation culture for 20h, inoculating the fermentation medium with the seed solution B, simultaneously refilling a nutrient solution, and continuing to implement fermentation culture for 40-50h, so that fermentation brothis obtained; centrifuging the fermentation broth at high speed, regulating pH value, and conducting extracting and concentrating, so as to a crude product; and implementing column chromatography and separating, so that the formamide phenazine bio-fungicide is obtained. The culture medium and culture conditions provided by the invention are quite suitable for growth of strains; the refilled nutrient solution contains chorismic acid; two strains undergo mixed fermentation; the content of formamide phenazine in the fermentation broth is obviously improved; based upon a series of post-treatment, afinished product that purity is higher than 99% is separated and obtained; and the finished product takes a strong inhibitory effect on pathogenic fungi of plants.

Description

technical field [0001] The invention belongs to the technical field of microbes, and relates to the preparation of biological bactericides, in particular to a method for preparing a caramiphenazine biological bactericide. Background technique [0002] Fungicides are a class of pesticides used to prevent and control plant diseases caused by various pathogenic microorganisms, generally referring to fungicides. [0003] Different fungicides work in different ways. The fungicide applied to the surface of the plant before the pathogen infestation has a preventive and protective effect is called a protective fungicide or a protective agent; the fungicide that can eliminate the infected bacteria at the application site is called an eradicating fungicide; Those that are transmitted to the infected part of the body to eliminate the germs are called systemic fungicides. Many eradicating agents are also systemic agents, and most of them have chemical therapeutic effects. In practice,...

Claims

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Application Information

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IPC IPC(8): C12P17/12A01N43/60A01P3/00C12R1/38C12R1/385
CPCA01N43/60C12P17/12
Inventor 徐福祥
Owner JIANGXI POROR CROPS ENG
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