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Culture method of Taishan codonopsis lanceolata micro-seminal root

A cultivation method and technology of four-leaf ginseng are applied in the field of agricultural biology to achieve the effects of short seedling slowing time, easy operation, convenient storage and long-distance transportation

Inactive Publication Date: 2019-01-25
TAISHAN RES INST OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the reported hardening and transplanting technology of Taishan four-leaf ginseng tissue culture seedlings is mainly the conventional transplanting technology of complete green seedlings with young tender roots, stems and leaves carried out by Li Tiejun et al. Combining with field rhizome transplanting technology, there is no public report on the discussion of Taishan four-leaf ginseng field micro-root propagation method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] From the Taishan four-leaf ginseng tissue-cultured seedlings, a single aseptic bottle seedling with robust growth, at least one node, thick stem, a base diameter of more than 0.2 cm, a height of more than 1 cm, and complete leaves was selected as the test material ;

[0034] Put the aseptic seedlings of Taishan four-leaf ginseng with the base downward, and cuttages on the sterile microroot induction medium to induce adventitious roots. The distance between the plants in the density bottle is 0.5-1.0 cm, and the height of each plant is 1-1.5 cm; the medium is used without any After 10-15 days of culture in the hormone-supplemented MS medium, white adventitious roots grow from the base of the stem segment, the number is 3-8, and the root diameter is about 0.1 cm;

[0035] After the rooted seedlings were continuously cultured on the same medium for 50-80 days, the white adventitious roots swelled and thickened obviously, and grew to a diameter of about 0.3 cm, and initiall...

Embodiment 2

[0043] From the Taishan four-leaf ginseng tissue-cultured seedlings, a single aseptic bottle seedling with robust growth, at least one node, thick stem, a base diameter of more than 0.2 cm, a height of more than 1 cm, and complete leaves was selected as the test material ;

[0044] The aseptic seedlings of Taishan four-leaf ginseng, with the base facing down, were cuttages in sterile microroot induction medium for adventitious root induction. The distance between plants in the density bottle is 0.5-1 cm, and the height of each plant is 1-1.5 cm. The culture medium is MS medium supplemented with IBA0.1-0.5 mg / L; after 7-15 days of culture, adventitious roots grow from the base of the stem segment, the number is 1-10, white, the length is 0.5-3 cm, and the root diameter is about 0.1 cm . After continuing to culture on the original medium for 40-60 days, the white adventitious roots at the base of the stem segment obviously swelled and thickened, and grew into a round or oval m...

Embodiment 3

[0052] From the Taishan four-leaf ginseng tissue-cultured seedlings, a single aseptic bottle seedling with robust growth, at least one node, thick stem, a base diameter of more than 0.2 cm, a height of more than 1 cm, and complete leaves was selected as the test material ;

[0053] Cut a 1-1.5 cm stem section with internodes and leaves from a sterile individual plant, with the base down, and insert the cuttings in the adventitious root induction medium according to the spacing of 0.5-1 cm in the bottle to induce adventitious roots to obtain rooted seedlings; sterile The microroot induction medium adopts MS medium and adds IBA0.1-0.5 mg / L; after 7-15 days of culture, adventitious roots grow from the base of the stem segment, the number is 1-10, white, the length is 0.5-3 cm, and the root diameter is 0.5-3 cm. About 0.1 cm; this type of rooted seedlings can be directly used for hardening and transplanting in experimental sites.

[0054] Select the sandy loam plot rich in humus ...

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Abstract

The invention discloses a culture method of a Taishan codonopsis lanceolata micro-seminal root. Directly transplanted rhizophorous micro-rhizomes are acquired by six steps, and field micro-seminal roots are further acquired. The micro-seminal roots have the remarkable advantages that the roots are small in size, convenient to transplant and store, long-distance transportation of the roots is facilitated, growth and development cycles are completely consistent with these of conventional field seedlings and the like. By the method, annual seedlings with excellent inheritable characters can be rapidly produced, an integrated breeding system from bottle seedlings to transplanting seedlings is formed, and the cultural method is a novel and efficient seedling breeding method. The culture methodfills the technical gaps of sterility micro-rhizome cultural of Taishan codonopsis lanceolata tissue culture seedlings and a breeding mode of field micro-seminal roots.

Description

technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to a method for cultivating micro-roots of Taishan four-leaf ginseng. Background technique [0002] Taishan four-leaf ginseng ( Codonopsis lanceolata (Sieb. et Zucc.) Trautv. ) for Platycodonaceae ( Campanulaceae ) Codonopsis ( Codonopsis ) Perennial herbaceous vines, also known as goat's milk, goat's milk ginseng, Codonopsis codonopsis, Taishan ginseng, etc.; mostly born in mountain shrubs, ditch wetlands and broad-leaved forests. Taishan four-leaf ginseng is mainly used as medicine with roots, which are sweet, pungent and flat. Return lung, liver, spleen and large intestine meridian. It has the effects of invigorating deficiency and clearing milk, draining pus and detoxifying. For physical weakness after illness, insufficient milk, mastitis, lung abscess, carbuncle furuncle sore throat moth embolism. [0003] The "Four Famous Medicines of Mount Tai" are character...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G22/25
CPCA01H4/008A01G22/25A01H4/001
Inventor 黄艳艳郭伟李国华王玉山赵进红王新花季霞李冬梅
Owner TAISHAN RES INST OF FORESTRY
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