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Method for constructing liver cancer patient-derived chick chorioallantoic membrane M-PDX model

A technology of chorioallantoic membrane and construction method, which is applied in the field of construction of M-PDX model of chicken embryo chorioallantoic membrane derived from liver cancer patients, can solve the problems of high price, long test period, animal ethics restrictions, etc., and achieve successful modeling The effect of high rate, short experiment period and convenient operation

Pending Publication Date: 2019-02-01
广州星燎生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the relatively strict requirements for feeding and breeding of nude mice, the price is relatively high, and the test period is long, the relevant nude mouse transplanted tumor models have certain limitations and are restricted by animal ethics.

Method used

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  • Method for constructing liver cancer patient-derived chick chorioallantoic membrane M-PDX model
  • Method for constructing liver cancer patient-derived chick chorioallantoic membrane M-PDX model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The method for constructing a chicken chorioallantoic membrane M-PDX model derived from liver cancer patients of the present invention includes four steps of clinical sample transfer, cryopreservation, recovery and transplantation;

[0033] S1. Obtain a clinical liver cancer tumor sample with a tissue mass of 150 mg from the operating room, and then place the clinical liver cancer tumor sample in a biopsy tissue culture medium; the formula of the biopsy tissue culture medium is: basal growth medium 500ml, 125,000 units of penicillin, 125,000 units of streptomycin, 50 mg of kanamycin, and 25 mg of gentamicin, and the clinical liver cancer tumor sample was kept in the biopsy tissue culture medium for 1 day;

[0034] S2. The steps of freezing include:

[0035] S 21 .Remove the necrotic tissue, fat and fibrous tissue of the clinical liver cancer tumor sample, then cut into 1.5mm thin slices, place it in the biopsy tissue culture medium for immersion, take out,

[0036] S ...

Embodiment 2

[0048] The method for constructing a chicken chorioallantoic membrane M-PDX model derived from a liver cancer patient in Example 2 includes four steps of clinical sample transfer, cryopreservation, resuscitation, and transplantation;

[0049] S1. Obtain a clinical liver cancer tumor sample with a tissue mass of 100 mg of liver S6 liver cancer tissue from the operating room, and then place the clinical liver cancer tumor sample in a biopsy tissue culture medium; the formula of the biopsy tissue culture medium is: 500 ml of basal growth medium , 125,000 units of penicillin, 125,000 units of streptomycin, 50 mg of kanamycin, and 25 mg of gentamicin, and the clinical liver cancer tumor sample is kept in the biopsy tissue culture medium for 2 days;

[0050] S2. The steps of freezing include:

[0051] S 21.Remove the necrotic tissue, fat and fibrous tissue of the clinical liver cancer tumor sample, then cut into 2mm thin slices, soak in the biopsy tissue culture medium, take out, ...

Embodiment 3

[0064] The method for constructing a chicken chorioallantoic membrane M-PDX model derived from a liver cancer patient in Example 3 includes four steps of clinical sample transfer, cryopreservation, resuscitation, and transplantation;

[0065] S1. Obtain a clinical liver cancer tumor sample with a mass of 300 mg of bulky liver cancer tissue from the operating room, first place the clinical liver cancer tumor sample in 70% alcohol for 30 seconds, and sterilize it, and then place the clinical liver cancer tumor sample in a biopsy tissue In the culture medium; the formula of the biopsy tissue culture medium is: 500ml of basal growth medium, 125000 units of penicillin, 125000 units of streptomycin, 50mg of kanamycin, and 25mg of gentamicin. Retain in the culture medium of described biopsy tissue for 1 day;

[0066] S2. The steps of freezing include:

[0067] S 21 .Remove the necrotic tissue, fat and fibrous tissue of the clinical liver cancer tumor sample, then cut into thin slic...

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Abstract

The invention discloses a method for constructing a liver cancer patient-derived chick chorioallantoic membrane M-PDX model. The method comprises the following steps: clinical sample transport, cryopreservation, resuscitation and transplantation. A human hepatic transplantable tumor and an angiogenesis model thereof are established on the chick chorioallantoic membrane (CAM) by using human hepatoma cells with poor differentiation and high metastasis to provide a simple and practical way for the study of hepatic transplantable tumor and angiogenesis thereof. Compared with conventional models, the model has the unique advantages of short experimental period, low cultivation cost, and convenience in operation.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for constructing an M-PDX model of chicken embryo chorioallantoic membrane derived from liver cancer patients. Background technique [0002] Anti-angiogenesis is one of the approaches in the treatment of liver cancer, and the establishment of angiogenesis models is a prerequisite for the study of liver cancer angiogenesis and the screening of angiogenesis inhibitors. In the traditional PDX model, the tumor carrier is generally nude mice, and the transplanted tumors in nude mice can preserve the original histological structure or various functions, and can reproduce the original cancer structure that has disappeared in tissue culture. However, due to the relatively strict requirements for feeding and breeding nude mice, the price is relatively high, and the test period is long, the relevant nude mouse transplanted tumor models have certain limitations and are restricted by an...

Claims

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Application Information

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IPC IPC(8): C12N5/073C12N5/09
CPCC12N5/0604C12N5/0693C12N2509/00
Inventor 文皓旻
Owner 广州星燎生物科技有限公司
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