Staphylococcus drug resistance inducing method

Abstract

The invention discloses a staphylococcus drug resistance inducing method. The staphylococcus drug resistance inducing method comprises (1) selection of staphylococcus strains, (2) screening of antibiotics, (3) solution preparation of standard antibiotics, (4) measurement of minimal inhibitory concentration through a microdilution broth method, (5) passage induction, and (6) measurement of drug resistance of every generation of staphylococcus to cefazolin. According to the staphylococcus drug resistance inducing method, instruments and equipment required by experimental operation can be reduced, and subsequent material processing difficulty can be greatly reduced. Therefore, the staphylococcus drug resistance inducing method can well solve the problems on operation difficulty and costs, andmeanwhile improve the induction efficiency, accuracy and success rate. Compared with other methods, the staphylococcus drug resistance inducing method is more convenient to operate and easier to grasp.

Description

technical field [0001] The invention belongs to the technical field of biopharmaceuticals, in particular to a method for inducing staphylococcus to produce drug resistance. Background technique [0002] At present, there are more and more drug-resistant bacteria, but in the past two decades, no new antibiotics have been produced. Now in life, the use of antibiotics has not been well regulated, and the drug-resistant bacteria studied now Most of them are isolated and purified from disease departments or nature, and their drug resistance analysis requires a lot of money and specimens, and it is difficult to analyze the variation process clearly. Therefore, the research on drug resistance of artificial microorganisms is even more urgent. The demand for bacterial materials is also greater. [0003] At present, there are mainly methods for inducing bacterial resistance: 1) Physical methods, such as gamma ray induction and ultraviolet irradiation. The mutations induced by this in...

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Problems solved by technology

[0002] At present, there are more and more drug-resistant bacteria, and in the past two decades, no new antibiotics have been produced. Now, the use of antibiotics in daily life has not been well regulated, and the drug-resistant bacteria studied now Most of them are isolated and purified from disease departments or nature, and their drug resistance analysis requires a lot of money and specimens, and it is difficult to analyze the variation process clearly. Therefore, the research on the drug resistance of artificial microorganisms is more urgent. The demand for bacterial materials is also greater

[0003] At present, the methods for inducing bacterial resistance mainly include: 1) Physical methods, such as gamma ray induction and ultraviolet irradiation. The mutations induced by this induction method are not directional, and further screening is required in the follow-up, and the radiation dose is not good. 2) chemical methods, such as drug concentration gradient plate method, this method needs to prepare a large number of samples, and the operation error control is also difficult It is more difficult, the space occupation rate is also high, the material consumption is relatively large, and the post-processing of materials is not enough; 3) biological methods, such as genetic engineering, this technology can solve the problem of non-directional variation to a large extent, but its operation cost High, technically demanding and cumbersome to operate

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