Reagent kit and method for quickly and efficiently detecting polymorphism of drug-induced skin adverse reaction related genes

Abstract

The invention discloses a reagent kit and a method for quickly and efficiently detecting the polymorphism of drug-induced skin adverse reaction related genes, and belongs to the technical field of gene detection. The polymorphism of the related genes includes the polymorphism of HLA-B*1502 and HLA-B*5801 genes. The reagent kit comprises sample treatment reagents, PCR (polymerase chain reaction) 8joint management individual-portion premixed and separately packaged gene detection reagents, positive reference substances and a negative reference substance. The sample treatment reagents are used for treating human whole-blood samples, the positive reference substances are gene plasmid DNA (deoxyribonucleic acid) and internal reference plasma DNA, and the negative reference substance is ultrapure water without target genes or internal reference genes. The reagent kit and the method have the advantages that specific amplification and real-time detection and analysis are carried out by specific amplification primer pairs and MGB-NFQ (minor groove binder-non-fluorescent quencher) fluorescent probes which are designed according to target gene sequences, accordingly, the detection specificity and sensitivity can be enhanced, and variation of desired genes can be accurately, specifically and sensitively detected from the polymorphism of thousands of allelic genes of HLA-B.

Description

technical field [0001] The invention relates to a kit and method for gene polymorphism, in particular to a kit and method for rapidly and efficiently detecting gene polymorphism related to drug-induced skin adverse reactions, belonging to the technical field of gene detection. Background technique [0002] Research studies have shown that drugs such as carbamazepine and phenytoin are used in the treatment of severe skin reactions of Stevens-Johnson syndrome sJs and toxic epidermal necrolysis TEN in HLA-B*1502 allele-positive Asian patients. According to preliminary data, HLA-B*1502 is a human leukocyte antigen HLA allele that exists almost exclusively in Asian races, including Chinese Han, Filipinos, Malaysians, South Asian Indians, and Thais. [0003] An estimated 10%-15% or more of patients in China, Thailand, Malaysia, Indonesia, the Philippines, and Taiwan carry the HLA-B*1502 allele, with moderate rates among South Asians, including Indians , The average is 2%-4%, but ...

AI Technical Summary

Problems solved by technology

[0007] The main purpose of the present invention is to provide a kit and method for quickly and efficiently detecting gene polymorphisms related to drug-induced skin adverse reactions, so as to solve the problem of detecting HLA-B*1502 and HLA-B*5801 gene polymorphisms in the prior art However, there are many problems such as high requirements for sample processing, long detection cycle, complicated operation, high cost and low specificity.

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