A controllable transdermal drug delivery preparation based on framework nucleic acid and its preparation method
A drug delivery and nucleic acid technology, which can be applied to pharmaceutical formulations, medical preparations with inactive ingredients, and medical preparations containing active ingredients, etc. Uncontrollable depth and other problems, to achieve the effect of solving the uncontrollable shape, size and penetration depth and good stability
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0046] Embodiment 1: Preparation of framework nucleic acid
[0047] Provide framework nucleic acid: DNA single strands are dissolved in a buffer solution for assembly. Specifically, DNA single strands are mixed in a certain proportion, and Mg-containing 2+ In the synthesis buffer, mix well, anneal, self-assemble to form framework nucleic acid.
[0048] Framework nucleic acid fluorescent labeling: obtained by extending the DNA single strand at the corresponding position and incubating with the complementary DNA single strand of the modified fluorescent molecule at room temperature for hybridization.
[0049] In this embodiment, eight kinds of framework nucleic acids with different structures, sizes and dimensions are provided, as shown in Table 1.
[0050] Table 1 Eight framework nucleic acids and their corresponding fluorescent labels
[0051]
Embodiment 2
[0052] Example 2: Verification of the morphology and size of the framework nucleic acid
[0053] (1) Dimensional measurement of the structure
[0054] The hydrodynamic diameter (Dh) was measured with a particle size analyzer Zetasizernano Z (Malvern), and the measurement was repeated at least three times. The result is as figure 1 As shown, the measured tetrahedral nanostructures: the average hydrodynamic diameters (Dh) of TH21, TH37 and TH337 are ~ (approximately, the same below) 17nm, ~44nm and ~187nm (theoretical sizes are respectively: ~7nm , ~12nm, ~100nm). Two six-helix rod-like nanostructures: 6H714 and 6H14498 have two peaks, corresponding to width ~ 30nm and rod length ~ 66 / 220nm (theoretical dimensions are: width ~ 6nm, rod length ~ 20nm / 400nm). The remaining larger rectangular or triangular nanostructures: R13730, B14498 and T14498 have similar average Dh, between 140-170nm (theoretical dimensions are: ~70×100nm, ~6×40×60nm, ~120nm side length).
[0055] (2) Ele...
Embodiment 3
[0057] Example 3: Observation of cellular uptake behavior of framework nucleic acids
[0058] (1) Cell culture and cell viability detection:
[0059] Normal dermal fibroblasts (normal dermal fibroblast or NDF) (purchased from CellResearch Corporation Pte Ltd, Singapore) and immortalized keratinocytes HaCaT (purchased from American Type Culture Collection, ATCC) were cultured in 10% fetal bovine serum (PBS), 1% gelatin serine (PS), 4mM L-glutamine (L-glutamine) in the DMEM medium (4500mg / L), 37 ℃, 5% CO 2 , cultured in saturated humidity, adding fresh medium every 2-3 days.
[0060] Alamarblue reagent was added to the medium at a volume ratio of 1:100, and after incubation for 8 hours, fluorescence measurement (570 / 585nm) was performed to detect cell viability.
[0061] (2) To study the influence of incubation time on the cellular uptake behavior of TH21 DNA tetrahedral nanostructures:
[0062] Fluorescence-labeled TH21 was added to normal skin fibroblasts at a final concent...
PUM
| Property | Measurement | Unit |
|---|---|---|
| molecular weight | aaaaa | aaaaa |
| molecular weight | aaaaa | aaaaa |
| molecular weight | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More