Method for cultivating selenium-enriched Cordyceps militaris

A culturing method and a technology for Cordyceps militaris, which are applied in the field of Cordyceps militaris cultivation, can solve the problem of low selenium-enriched content of Cordyceps militaris, and achieve the effects of inducing absorption and accumulation, increasing content, and improving absorption and accumulation.

Inactive Publication Date: 2019-03-01
安徽祥康生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] For this reason, the technical problem to be solved by the present invention is to provide a kind of cultivation method of selenium-enriched Cordyceps militaris, to solve the problem that the selenium-enriched amount of Cordyceps militaris is slightly low in the prior art

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  • Method for cultivating selenium-enriched Cordyceps militaris
  • Method for cultivating selenium-enriched Cordyceps militaris

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Embodiment 1

[0020] The cultivation method of the selenium-enriched Cordyceps militaris described in this embodiment includes carrying out strain activation in the strain activation medium, carrying out seed liquid culture in the seed liquid medium, and carrying out fermenter bacteria in the mycelia fermentation medium. Steps in filament culture.

[0021] The bacterial strain activation medium described in this embodiment comprises the following components in mass content: take the above-mentioned components according to the ratio of 1% glucose, 3% sucrose, 2% peptone, and 1.5% egg white; respectively take 10 g of glucose, 30 g of sucrose, and 20 g of peptone 1. Egg white 15g, add water to make slurry and make it to 1L, natural pH, sterilize at 121°C for 20min, then divide into test tubes, insert Cordyceps militaris strains into test tubes under aseptic conditions, at 20°C, speed 150rpm Carry out dark activation culture for 2-3 days.

[0022] The seed liquid culture medium described in th...

Embodiment 2

[0025] The cultivation method of the selenium-enriched Cordyceps militaris described in this embodiment includes carrying out strain activation in the strain activation medium, carrying out seed liquid culture in the seed liquid medium, and carrying out fermenter bacteria in the mycelia fermentation medium. Steps in filament culture.

[0026] The bacterial strain activation medium described in this embodiment comprises the following components in mass content: take the above-mentioned components according to the ratio of 3% glucose, 1% sucrose, 4% peptone, and 0.8% egg white; respectively take 30g of glucose, 10g of sucrose, and 40g of peptone 1. Egg white 8g, add water to make slurry and make it to 1L, natural pH, sterilize at 121°C for 20min, then pack into test tubes, put Cordyceps militaris strains into test tubes under aseptic conditions, at 25°C, speed 150rpm Carry out dark activation culture for 2-3 days.

[0027] The seed liquid culture medium described in this embodi...

Embodiment 3

[0030] The cultivation method of the selenium-enriched Cordyceps militaris described in this embodiment includes carrying out strain activation in the strain activation medium, carrying out seed liquid culture in the seed liquid medium, and carrying out fermenter bacteria in the mycelia fermentation medium. Steps in filament culture.

[0031]The bacterial strain activation medium described in this embodiment comprises the following components in mass content: take the above-mentioned components according to the ratio of glucose 2%, sucrose 2%, peptone 3%, and egg white 1%; take glucose 20g, sucrose 20g, and peptone 30g respectively 1. Egg white 10g, add water to make slurry and make it to 1L, natural pH, sterilize at 121°C for 20min, then pack into test tubes, put Cordyceps militaris strains into test tubes under aseptic conditions, at 22°C, speed 150rpm Carry out dark activation culture for 2-3 days.

[0032] The seed liquid culture medium described in this embodiment includ...

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Abstract

The invention belongs to the technical field of cultivation of Cordyceps militaris, in particular to a method for cultivating selenium-enriched Cordyceps militaris. According to the method for cultivating the selenium-enriched Cordyceps militaris, a strain activation culture medium, a seed liquid culture medium and a liquid fermentation medium are carefully screened, molasses, sucrose, peptone, radix astragali, yeast selenium, radish, Enteromorpha residue, egg white, potassium dihydrogen phosphate and magnesium sulfate are taken as main nutrient raw materials, and cathepsin and cellulase are added, so that dissolution and absorption of various raw materials are facilitated; and added EDTA-4Na also contributes to transfer and absorption of selenium. Furthermore, in the method of the presentinvention, the content of selenium in mycelia of Cordyceps militaris is further increased by adding amino acid chelated calcium to the fermentation medium to better induce absorption and accumulationof selenium.

Description

technical field [0001] The invention belongs to the technical field of Cordyceps militaris cultivation, and in particular relates to a method for cultivating selenium-enriched Cordyceps militaris. Background technique [0002] Cordyceps militaris (L.) Link, also known as Cordyceps militaris, belongs to Eumycota, Ascomycota, Ascomycotinia, Sordariomycetes, Hypocrea Type species of subclass Hypocreomycetidae, Hypocreales, Cordycipitaceae, Cordyceps. Cordyceps militaris usually has similar bioactive components to Cordyceps sinensis, mainly including cordycepin, cordycepic acid, polysaccharides, ergosterol, amino acids, nucleosides, etc. The efficacy of Cordyceps militaris is also similar to that of Cordyceps sinensis, and it is a good substitute for Cordyceps sinensis. Cordyceps militaris is a natural resource, showing a worldwide distribution, but the quantity is very small, and most rely on artificial cultivation. In recent years, humans have conducted extensive research on...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/20
CPCA01G18/20
Inventor 詹怀敬杨军颜迟贾俊杰周兰
Owner 安徽祥康生物工程有限公司
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