A kind of method for prolonging the lifespan of adult beetles
A beetle and adult technology, applied in the direction of animal husbandry and the like, can solve the problems of shortened lifespan, decreased egg production, affected egg production, etc., and achieves the effects of increasing adult lifespan, improving reproduction and saving economy.
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Embodiment 1
[0035] A method for prolonging the lifespan of adult stinkbugs, comprising the following steps: 1) putting 3 to 5 grams of newly produced stinkbug eggs into a 50ml centrifuge tube; 2) adding untreated stinkbug eggs to the centrifuge tube in step 1) After sterilizing the eggs with deionized water, put the centrifuge tube into the shaker for three times of shaking, each time shaking at a speed of 700-800rpm for 15s, and let the centrifuge tube stand for 3 minutes between two adjacent shaking treatments; then centrifuge Suck out the sterilized deionized water in the tube; then add a volume concentration of 75% ethanol solution submerged in the stinkbug eggs to the centrifuge tube, put the centrifuge tube into the oscillator for 15s, and shake at a speed of 700-800rpm. After standing still for 4min, the ethanol solution in the centrifuge tube was subsequently sucked out; 3) after the stinkbug ovum obtained in step 2) was rinsed with sterilized deionized water, the stinkbug ovum was...
Embodiment 2
[0037] A method for prolonging the lifespan of adult stinkbugs, comprising the following steps: 1) putting 3 to 5 grams of newly produced stinkbug eggs into a 50ml centrifuge tube; 2) adding untreated stinkbug eggs to the centrifuge tube in step 1) After sterilizing the eggs with deionized water, put the centrifuge tube into the oscillator for four shock treatments, each time at a speed of 700-800rpm for 15s, and let the centrifuge tube stand for 3 minutes between two adjacent shock treatments; Suck out the sterilized deionized water in the centrifuge tube; 3) Rinse the stinkbug ovum obtained in step 2) with sterilized deionized water, then place the stink bug ovum on sterilized filter paper to absorb the surface moisture ; 4) Place the stinkbug eggs obtained in step 3) evenly in a 12cm sterilized culture dish, set the temperature of the culture environment to be 25-27°C, the humidity to be 60-70%, and the photoperiod to be 16L:6D; The sterilized filter paper sprayed with ster...
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